A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: dissociation analysis of amplified fragments of DNA

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene : dissociation analysis of amplified fragments of DNA. / Rasmussen, Henrik Berg; Werge, Thomas.

I: Molecular and Cellular Probes, Bind 21, Nr. 1, 2007, s. 8-11.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rasmussen, HB & Werge, T 2007, 'A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: dissociation analysis of amplified fragments of DNA', Molecular and Cellular Probes, bind 21, nr. 1, s. 8-11. https://doi.org/10.1016/j.mcp.2006.05.003

APA

Rasmussen, H. B., & Werge, T. (2007). A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: dissociation analysis of amplified fragments of DNA. Molecular and Cellular Probes, 21(1), 8-11. https://doi.org/10.1016/j.mcp.2006.05.003

Vancouver

Rasmussen HB, Werge T. A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: dissociation analysis of amplified fragments of DNA. Molecular and Cellular Probes. 2007;21(1):8-11. https://doi.org/10.1016/j.mcp.2006.05.003

Author

Rasmussen, Henrik Berg ; Werge, Thomas. / A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene : dissociation analysis of amplified fragments of DNA. I: Molecular and Cellular Probes. 2007 ; Bind 21, Nr. 1. s. 8-11.

Bibtex

@article{da07f9d2e725461b81ca118375ca14c8,
title = "A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: dissociation analysis of amplified fragments of DNA",
abstract = "We have developed a closed-tube assay for determination of the chemokine receptor type 5 (CCR5) 32-bp deletion allele, which protects against infections with HIV and modulates susceptibility to a variety of inflammatory diseases. This assay utilizes dissociation analysis of amplified products in the presence of Sybr Green I for allele discrimination. After having established robust conditions for the assay, we used it to genotype 590 unknown DNA samples. A blinded comparison with a procedure based upon agarose gel electrophoresis of amplified material revealed complete concordance between the two procedures. Our closed-tube assay is inexpensive and easy to carry out. Furthermore, it reduces or eliminates the risk of carry-over contamination with previously amplified products. The insights gained in this study can be applied to develop assays for genotyping of other insertion/deletion polymorphisms based upon differences in T(m) of allele-specific amplicons.",
author = "Rasmussen, {Henrik Berg} and Thomas Werge",
year = "2007",
doi = "http://dx.doi.org/10.1016/j.mcp.2006.05.003",
language = "English",
volume = "21",
pages = "8--11",
journal = "Molecular and Cellular Probes",
issn = "0890-8508",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene

T2 - dissociation analysis of amplified fragments of DNA

AU - Rasmussen, Henrik Berg

AU - Werge, Thomas

PY - 2007

Y1 - 2007

N2 - We have developed a closed-tube assay for determination of the chemokine receptor type 5 (CCR5) 32-bp deletion allele, which protects against infections with HIV and modulates susceptibility to a variety of inflammatory diseases. This assay utilizes dissociation analysis of amplified products in the presence of Sybr Green I for allele discrimination. After having established robust conditions for the assay, we used it to genotype 590 unknown DNA samples. A blinded comparison with a procedure based upon agarose gel electrophoresis of amplified material revealed complete concordance between the two procedures. Our closed-tube assay is inexpensive and easy to carry out. Furthermore, it reduces or eliminates the risk of carry-over contamination with previously amplified products. The insights gained in this study can be applied to develop assays for genotyping of other insertion/deletion polymorphisms based upon differences in T(m) of allele-specific amplicons.

AB - We have developed a closed-tube assay for determination of the chemokine receptor type 5 (CCR5) 32-bp deletion allele, which protects against infections with HIV and modulates susceptibility to a variety of inflammatory diseases. This assay utilizes dissociation analysis of amplified products in the presence of Sybr Green I for allele discrimination. After having established robust conditions for the assay, we used it to genotype 590 unknown DNA samples. A blinded comparison with a procedure based upon agarose gel electrophoresis of amplified material revealed complete concordance between the two procedures. Our closed-tube assay is inexpensive and easy to carry out. Furthermore, it reduces or eliminates the risk of carry-over contamination with previously amplified products. The insights gained in this study can be applied to develop assays for genotyping of other insertion/deletion polymorphisms based upon differences in T(m) of allele-specific amplicons.

U2 - http://dx.doi.org/10.1016/j.mcp.2006.05.003

DO - http://dx.doi.org/10.1016/j.mcp.2006.05.003

M3 - Journal article

VL - 21

SP - 8

EP - 11

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

IS - 1

ER -

ID: 48610005