Identification of one capa and two pyrokinin receptors from the malaria mosquito Anopheles gambiae.
Research output: Contribution to journal › Journal article › Research › peer-review
We cloned the cDNA of three evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae and functionally expressed them in Chinese hamster ovary cells. One receptor, Ang-Capa-R, was only activated by the two Anopheles capa neuropeptides Ang-capa-1 (GPTVGLFAFPRVamide) and Ang-capa-2 (pQGLVPFPRVamide) with EC(50) values of 8.6x10(-9)M and 3.3x10(-9)M, respectively, but not by any other known mosquito neuropeptide. The second receptor, Ang-PK-1-R, was selectively activated by the Anopheles pyrokinin-1 peptides Ang-PK-1-1 (AGGTGANSAMWFGPRLamide) and Ang-PK-1-2 (AAAMWFGPRLamide) with EC(50) values of 3.3x10(-8)M and 2.5x10(-8)M, respectively, but not by mosquito capa or pyrokinin-2 peptides. For the third receptor, Ang-PK-2-R, the most potent ligands were the pyrokinin-2 peptides Ang-PK-2-1 (DSVGENHQRPPFAPRLamide) and Ang-PK-2-2 (NLPFSPRLamide) with EC(50) values of 5.2x10(-9)M and 6.4x10(-9)M, respectively. However, this receptor could also be activated by the two pyrokinins-1, albeit with lower potency (EC(50): 2-5x10(-8)M). Because Ang-capa-1 and -2 and Ang-PK-1-1 are located on one preprohormone and the other peptides on another prohormone, these results imply a considerable crosstalk between the capa, pyrokinin-1 and pyrokinin-2 systems. Gene structure and phylogenetic tree analyses showed that Ang-Capa-R was the orthologue of the Drosophila capa receptor CG14575, Ang-PK-1-R the orthologue of the Drosophila pyrokinin-1 receptor CG9918, and Ang-PK-2-R the orthologue of the Drosophila pyrokinin-2 receptors CG8784 and CG8795. This is the first report on the functional characterization and crosstalk properties of capa and pyrokinin receptors in mosquitoes.
Original language | English |
---|---|
Journal | Biochemical and Biophysical Research Communications |
Volume | 362 |
Issue number | 2 |
Pages (from-to) | 245-51 |
Number of pages | 6 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 2007 |
Bibliographical note
Keywords: Amino Acid Sequence; Animals; Anopheles gambiae; CHO Cells; Cricetinae; Cricetulus; DNA, Complementary; GTP-Binding Proteins; Green Fluorescent Proteins; Humans; Insect Proteins; Insect Vectors; Malaria; Molecular Sequence Data; Neuropeptides; Phylogeny; Receptor Cross-Talk; Receptors, G-Protein-Coupled; Receptors, Neuropeptide; Sequence Alignment; Sequence Homology, Amino Acid; Transfection
ID: 3045690