Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus
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The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.
Original language | English |
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Article number | 103348 |
Journal | Stem Cell Research |
Volume | 76 |
Number of pages | 5 |
ISSN | 1873-5061 |
DOIs | |
Publication status | Published - 2024 |
Bibliographical note
Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
ID: 384067102