Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017
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Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017. / Joensen, Katrine G.; Kiil, Kristoffer; Gantzhorn, Mette R.; Nauerby, Birgitte; Engberg, Jørgen; Holt, Hanne M.; Nielsen, Hans L.; Petersen, Andreas M.; Kuhn, Katrin G.; Sandø, Gudrun; Ethelberg, Steen; Nielsen, Eva M.
In: Emerging Infectious Diseases , Vol. 26, No. 3, 2020, p. 523-532.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017
AU - Joensen, Katrine G.
AU - Kiil, Kristoffer
AU - Gantzhorn, Mette R.
AU - Nauerby, Birgitte
AU - Engberg, Jørgen
AU - Holt, Hanne M.
AU - Nielsen, Hans L.
AU - Petersen, Andreas M.
AU - Kuhn, Katrin G.
AU - Sandø, Gudrun
AU - Ethelberg, Steen
AU - Nielsen, Eva M.
PY - 2020
Y1 - 2020
N2 - In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3%) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54%) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.
AB - In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3%) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54%) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.
U2 - 10.3201/eid2603.190947
DO - 10.3201/eid2603.190947
M3 - Journal article
C2 - 32091364
AN - SCOPUS:85079910115
VL - 26
SP - 523
EP - 532
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
SN - 1080-6040
IS - 3
ER -
ID: 244915054