TR146 cells grown on filters as a model of human buccal epithelium: III. Permeability enhancement by different pH values, different osmolality values, and bile salts
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TR146 cells grown on filters as a model of human buccal epithelium : III. Permeability enhancement by different pH values, different osmolality values, and bile salts. / Nielsen, Hanne Mørck; Rassing, M R.
In: International Journal of Pharmaceutics, Vol. 185, No. 2, 1999, p. 215-25.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - TR146 cells grown on filters as a model of human buccal epithelium
T2 - III. Permeability enhancement by different pH values, different osmolality values, and bile salts
AU - Nielsen, Hanne Mørck
AU - Rassing, M R
PY - 1999
Y1 - 1999
N2 - The aim of the present study was to evaluate the TR146 cell culture model as an in vitro model of human buccal epithelium with respect to the permeability enhancement by different pH values, different osmolality values or bile salts. For this purpose, the increase in the apparent permeability (P(app)) of the hydrophilic marker mannitol due to exposure to solutions with pH values or osmolality values different from the physiological values was studied. As in studies with solutions of either taurocholate (TC), glycocholate (GC) or glycodeoxycholate (GDC) the results were compared to the increase in P(app) of mannitol obtained in analog studies using porcine buccal mucosa in an Ussing chamber. The effect of the exposure on the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa was measured, and the degree of protein leakage due to GC exposure was investigated in the TR146 cell culture model. The porcine buccal mucosa was approximately ten times less permeable to mannitol than the TR146 cell culture model. The P(app)TC. Increased P(app) values correlated with a decrease in the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa. GC was shown to induce concentration dependent protein leakage in the TR146 cell culture but only from the site of application, and the results indicate that duration of exposure further than 120 min was of minor importance. The present results indicate that the TR146 cell culture model may be a suitable in vitro model for efficacy studies and mechanistic studies of enhancers with potential use in human buccal drug delivery.
AB - The aim of the present study was to evaluate the TR146 cell culture model as an in vitro model of human buccal epithelium with respect to the permeability enhancement by different pH values, different osmolality values or bile salts. For this purpose, the increase in the apparent permeability (P(app)) of the hydrophilic marker mannitol due to exposure to solutions with pH values or osmolality values different from the physiological values was studied. As in studies with solutions of either taurocholate (TC), glycocholate (GC) or glycodeoxycholate (GDC) the results were compared to the increase in P(app) of mannitol obtained in analog studies using porcine buccal mucosa in an Ussing chamber. The effect of the exposure on the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa was measured, and the degree of protein leakage due to GC exposure was investigated in the TR146 cell culture model. The porcine buccal mucosa was approximately ten times less permeable to mannitol than the TR146 cell culture model. The P(app)TC. Increased P(app) values correlated with a decrease in the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa. GC was shown to induce concentration dependent protein leakage in the TR146 cell culture but only from the site of application, and the results indicate that duration of exposure further than 120 min was of minor importance. The present results indicate that the TR146 cell culture model may be a suitable in vitro model for efficacy studies and mechanistic studies of enhancers with potential use in human buccal drug delivery.
KW - Animals
KW - Bile Acids and Salts
KW - Cell Culture Techniques
KW - Cell Division
KW - Cell Membrane Permeability
KW - Cheek
KW - Cholagogues and Choleretics
KW - Epithelial Cells
KW - Filtration
KW - Glycocholic Acid
KW - Glycodeoxycholic Acid
KW - Humans
KW - Hydrogen-Ion Concentration
KW - Mannitol
KW - Mouth Mucosa
KW - Osmolar Concentration
KW - Swine
KW - Taurocholic Acid
M3 - Journal article
C2 - 10460917
VL - 185
SP - 215
EP - 225
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 2
ER -
ID: 43890790