Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound. / Roursgaard, Martin; Poulsen, Steen Seier; Poulsen, Lars K.; Hammer, Maria; Jensen, Keld A.; Utsunomiya, Satoshi; Ewing, Rodney C.; Balic Zunic, Tonci; Nielsen, Gunnar D.; Larsen, Søren T.
In: Human & Experimental Toxicology, Vol. 29, No. 11, 2010, p. 915-933.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound
AU - Roursgaard, Martin
AU - Poulsen, Steen Seier
AU - Poulsen, Lars K.
AU - Hammer, Maria
AU - Jensen, Keld A.
AU - Utsunomiya, Satoshi
AU - Ewing, Rodney C.
AU - Balic Zunic, Tonci
AU - Nielsen, Gunnar D.
AU - Larsen, Søren T.
PY - 2010
Y1 - 2010
N2 - An increasing number of engineered particles, including nanoparticles, are being manufactured, increasing the need for simple low-dose toxicological screening methods. This study aimed to investigate the kinetics of biomarkers related to acute and sub-chronic particle-induced lung inflammation of quartz. Mice were intratracheal instilled with 50 µg of microsized or nanosized quartz. Acute inflammation was assessed 1, 2, 4, 8, 16 or 48 hours post exposure, whereas sub-chronic inflammation was investigated 3 months after exposure. Markers of acute inflammation in the bronchoalveolar lavage fluid (BALF) were neutrophils (PMN), tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1ß, macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC) and total protein, which were all close to maximum 16 hours post instillation. No major differences were seen in the time-response profiles of nano- and micro-sized particles. The potency of the two samples cannot be compared; during the milling process, a substantial part of the quartz was converted to amorphous silica and contaminated with corundum. For screening, BALF PMN, either TNF-a or IL-1ß at 16 hours post instillation may be useful. At 3 months post instillation, KC, PMN and macrophages were elevated. Histology showed no interstitial inflammation three months post instillation. For screening of sub-chronic effects, KC, PMN, macrophages and histopathology is considered sufficient.
AB - An increasing number of engineered particles, including nanoparticles, are being manufactured, increasing the need for simple low-dose toxicological screening methods. This study aimed to investigate the kinetics of biomarkers related to acute and sub-chronic particle-induced lung inflammation of quartz. Mice were intratracheal instilled with 50 µg of microsized or nanosized quartz. Acute inflammation was assessed 1, 2, 4, 8, 16 or 48 hours post exposure, whereas sub-chronic inflammation was investigated 3 months after exposure. Markers of acute inflammation in the bronchoalveolar lavage fluid (BALF) were neutrophils (PMN), tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1ß, macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC) and total protein, which were all close to maximum 16 hours post instillation. No major differences were seen in the time-response profiles of nano- and micro-sized particles. The potency of the two samples cannot be compared; during the milling process, a substantial part of the quartz was converted to amorphous silica and contaminated with corundum. For screening, BALF PMN, either TNF-a or IL-1ß at 16 hours post instillation may be useful. At 3 months post instillation, KC, PMN and macrophages were elevated. Histology showed no interstitial inflammation three months post instillation. For screening of sub-chronic effects, KC, PMN, macrophages and histopathology is considered sufficient.
KW - Animals
KW - Bronchoalveolar Lavage Fluid
KW - Cytokines
KW - Dose-Response Relationship, Drug
KW - Female
KW - Inhalation Exposure
KW - Mice
KW - Mice, Inbred BALB C
KW - Microscopy, Electron, Transmission
KW - Nanoparticles
KW - Neutrophils
KW - Particle Size
KW - Pneumonia
KW - Powder Diffraction
KW - Quartz
KW - Surface Properties
KW - Time Factors
KW - Toxicity Tests
KW - X-Ray Diffraction
U2 - 10.1177/0960327110363329
DO - 10.1177/0960327110363329
M3 - Journal article
C2 - 20237177
VL - 29
SP - 915
EP - 933
JO - Human and Experimental Toxicology
JF - Human and Experimental Toxicology
SN - 0960-3271
IS - 11
ER -
ID: 33717947