The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus. / Aaron, Michelle; Charbon, Godefroid; Lam, Hubert; Schwarz, Heinz; Vollmer, Waldemar; Jacobs-Wagner, Christine.
In: Molecular Microbiology, Vol. 64, No. 4, 05.2007, p. 938-952.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus
AU - Aaron, Michelle
AU - Charbon, Godefroid
AU - Lam, Hubert
AU - Schwarz, Heinz
AU - Vollmer, Waldemar
AU - Jacobs-Wagner, Christine
PY - 2007/5
Y1 - 2007/5
N2 - The tubulin homologue FtsZ is well known for its essential function in bacterial cell division. Here, we show that in Caulobacter crescentus, FtsZ also plays a major role in cell elongation by spatially regulating the location of MurG, which produces the essential lipid II peptidoglycan cell wall precursor. The early assembly of FtsZ into a highly mobile ring-like structure during cell elongation is quickly followed by the recruitment of MurG and a major redirection of peptidoglycan precursor synthesis to the midcell region. These FtsZ-dependent events occur well before cell constriction and contribute to cell elongation. In the absence of FtsZ, MurG fails to accumulate near midcell and cell elongation proceeds unperturbed in appearance by insertion of peptidoglycan material along the entire sidewalls. Evidence suggests that bacteria use both a FtsZ-independent and a FtsZ-dependent mode of peptidoglycan synthesis to elongate, the importance of each mode depending on the timing of FtsZ assembly during elongation.
AB - The tubulin homologue FtsZ is well known for its essential function in bacterial cell division. Here, we show that in Caulobacter crescentus, FtsZ also plays a major role in cell elongation by spatially regulating the location of MurG, which produces the essential lipid II peptidoglycan cell wall precursor. The early assembly of FtsZ into a highly mobile ring-like structure during cell elongation is quickly followed by the recruitment of MurG and a major redirection of peptidoglycan precursor synthesis to the midcell region. These FtsZ-dependent events occur well before cell constriction and contribute to cell elongation. In the absence of FtsZ, MurG fails to accumulate near midcell and cell elongation proceeds unperturbed in appearance by insertion of peptidoglycan material along the entire sidewalls. Evidence suggests that bacteria use both a FtsZ-independent and a FtsZ-dependent mode of peptidoglycan synthesis to elongate, the importance of each mode depending on the timing of FtsZ assembly during elongation.
KW - Bacterial Outer Membrane Proteins/analysis
KW - Bacterial Proteins/analysis
KW - Caulobacter crescentus/cytology
KW - Cell Wall/chemistry
KW - Cytoskeletal Proteins/analysis
KW - Microscopy, Confocal
KW - Microscopy, Fluorescence
KW - N-Acetylglucosaminyltransferases/analysis
KW - Silver Staining
KW - Uridine Diphosphate N-Acetylmuramic Acid/analogs & derivatives
U2 - 10.1111/j.1365-2958.2007.05720.x
DO - 10.1111/j.1365-2958.2007.05720.x
M3 - Journal article
C2 - 17501919
VL - 64
SP - 938
EP - 952
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 4
ER -
ID: 201156179