The impact of the biological variability or assay performance on AMH measurements: A prospective cohort study with AMH tested on three analytical assay-platforms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The impact of the biological variability or assay performance on AMH measurements : A prospective cohort study with AMH tested on three analytical assay-platforms. / Bungum, Leif; Tagevi, Julia; Jokubkiene, Ligita; Bungum, Mona; Giwercman, Aleksander; Macklon, Nick; Andersen, Claus Yding; Klausen, Tobias Wirenfeldt; Tørring, Niels; Kumar, Ajay; Skouby, Sven Olaf.

In: Frontiers in Endocrinology, Vol. 9, No. OCT, 595, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bungum, L, Tagevi, J, Jokubkiene, L, Bungum, M, Giwercman, A, Macklon, N, Andersen, CY, Klausen, TW, Tørring, N, Kumar, A & Skouby, SO 2018, 'The impact of the biological variability or assay performance on AMH measurements: A prospective cohort study with AMH tested on three analytical assay-platforms', Frontiers in Endocrinology, vol. 9, no. OCT, 595. https://doi.org/10.3389/fendo.2018.00603

APA

Bungum, L., Tagevi, J., Jokubkiene, L., Bungum, M., Giwercman, A., Macklon, N., Andersen, C. Y., Klausen, T. W., Tørring, N., Kumar, A., & Skouby, S. O. (2018). The impact of the biological variability or assay performance on AMH measurements: A prospective cohort study with AMH tested on three analytical assay-platforms. Frontiers in Endocrinology, 9(OCT), [595]. https://doi.org/10.3389/fendo.2018.00603

Vancouver

Bungum L, Tagevi J, Jokubkiene L, Bungum M, Giwercman A, Macklon N et al. The impact of the biological variability or assay performance on AMH measurements: A prospective cohort study with AMH tested on three analytical assay-platforms. Frontiers in Endocrinology. 2018;9(OCT). 595. https://doi.org/10.3389/fendo.2018.00603

Author

Bungum, Leif ; Tagevi, Julia ; Jokubkiene, Ligita ; Bungum, Mona ; Giwercman, Aleksander ; Macklon, Nick ; Andersen, Claus Yding ; Klausen, Tobias Wirenfeldt ; Tørring, Niels ; Kumar, Ajay ; Skouby, Sven Olaf. / The impact of the biological variability or assay performance on AMH measurements : A prospective cohort study with AMH tested on three analytical assay-platforms. In: Frontiers in Endocrinology. 2018 ; Vol. 9, No. OCT.

Bibtex

@article{7f1f7636bacb4be8966953cecf44170c,
title = "The impact of the biological variability or assay performance on AMH measurements: A prospective cohort study with AMH tested on three analytical assay-platforms",
abstract = "This study examined longitudinal, age-related and intra-individual variation in Anti-M{\"u}llerian Hormone (AMH) in regular menstruating women and correlated the hormonal levels to the antral follicle count (AFC). The impact of variations on an algorithm for calculation of follitropin-dose for ovarian stimulation were also tested. The study was carried out at a fertility clinic of a tertiary university hospital and had a prospective trial design. Twenty-six healthy women not receiving infertility treatment aged 22 to 50 years participated. Blood sampling for hormonal analysis was done every fifth day throughout three consecutive menstrual cycles, AFC was determined with 3-dimentional ultrasound and AMH measured by different assays from Beckman Coulter, Roche and Ansh Labs. Outcome measures were maximum and minimum difference in absolute and relative terms for each study subject during the test-period, coefficient of variation (Cv) for AMH for each cycle and cycle-day and correlation between AMH and AFC. The impact from variable AMH levels on an algorithm calculating follitrophin-delta dose in ovarian stimulation was explored. A significant longitudinal age-independent variation in AMH-levels and coefficient of variation in cycles and cycle days was found. A strong correlation between AMH-levels and AFC was confirmed and a case of significant divergence between assays was seen. Variations in AMH had a significant impact on an algorithm calculated dosage of gonadotrophins in ovarian stimulation. The finding of a substantial longitudinal variation in AMH question one recording being sufficient in quantifying gonadotrophins for ovarian stimulation, decision making and prognostication related to infertility treatment and counseling. Occasionally, commercial assays may fail to recognize specific AMH cleavage-products.",
keywords = "Anti mullerian hormone, Antral follicle count, Biomarkers, Female infertility, Infertility counseling",
author = "Leif Bungum and Julia Tagevi and Ligita Jokubkiene and Mona Bungum and Aleksander Giwercman and Nick Macklon and Andersen, {Claus Yding} and Klausen, {Tobias Wirenfeldt} and Niels T{\o}rring and Ajay Kumar and Skouby, {Sven Olaf}",
year = "2018",
doi = "10.3389/fendo.2018.00603",
language = "English",
volume = "9",
journal = "Frontiers in Endocrinology",
issn = "1664-2392",
publisher = "Frontiers Media S.A.",
number = "OCT",

}

RIS

TY - JOUR

T1 - The impact of the biological variability or assay performance on AMH measurements

T2 - A prospective cohort study with AMH tested on three analytical assay-platforms

AU - Bungum, Leif

AU - Tagevi, Julia

AU - Jokubkiene, Ligita

AU - Bungum, Mona

AU - Giwercman, Aleksander

AU - Macklon, Nick

AU - Andersen, Claus Yding

AU - Klausen, Tobias Wirenfeldt

AU - Tørring, Niels

AU - Kumar, Ajay

AU - Skouby, Sven Olaf

PY - 2018

Y1 - 2018

N2 - This study examined longitudinal, age-related and intra-individual variation in Anti-Müllerian Hormone (AMH) in regular menstruating women and correlated the hormonal levels to the antral follicle count (AFC). The impact of variations on an algorithm for calculation of follitropin-dose for ovarian stimulation were also tested. The study was carried out at a fertility clinic of a tertiary university hospital and had a prospective trial design. Twenty-six healthy women not receiving infertility treatment aged 22 to 50 years participated. Blood sampling for hormonal analysis was done every fifth day throughout three consecutive menstrual cycles, AFC was determined with 3-dimentional ultrasound and AMH measured by different assays from Beckman Coulter, Roche and Ansh Labs. Outcome measures were maximum and minimum difference in absolute and relative terms for each study subject during the test-period, coefficient of variation (Cv) for AMH for each cycle and cycle-day and correlation between AMH and AFC. The impact from variable AMH levels on an algorithm calculating follitrophin-delta dose in ovarian stimulation was explored. A significant longitudinal age-independent variation in AMH-levels and coefficient of variation in cycles and cycle days was found. A strong correlation between AMH-levels and AFC was confirmed and a case of significant divergence between assays was seen. Variations in AMH had a significant impact on an algorithm calculated dosage of gonadotrophins in ovarian stimulation. The finding of a substantial longitudinal variation in AMH question one recording being sufficient in quantifying gonadotrophins for ovarian stimulation, decision making and prognostication related to infertility treatment and counseling. Occasionally, commercial assays may fail to recognize specific AMH cleavage-products.

AB - This study examined longitudinal, age-related and intra-individual variation in Anti-Müllerian Hormone (AMH) in regular menstruating women and correlated the hormonal levels to the antral follicle count (AFC). The impact of variations on an algorithm for calculation of follitropin-dose for ovarian stimulation were also tested. The study was carried out at a fertility clinic of a tertiary university hospital and had a prospective trial design. Twenty-six healthy women not receiving infertility treatment aged 22 to 50 years participated. Blood sampling for hormonal analysis was done every fifth day throughout three consecutive menstrual cycles, AFC was determined with 3-dimentional ultrasound and AMH measured by different assays from Beckman Coulter, Roche and Ansh Labs. Outcome measures were maximum and minimum difference in absolute and relative terms for each study subject during the test-period, coefficient of variation (Cv) for AMH for each cycle and cycle-day and correlation between AMH and AFC. The impact from variable AMH levels on an algorithm calculating follitrophin-delta dose in ovarian stimulation was explored. A significant longitudinal age-independent variation in AMH-levels and coefficient of variation in cycles and cycle days was found. A strong correlation between AMH-levels and AFC was confirmed and a case of significant divergence between assays was seen. Variations in AMH had a significant impact on an algorithm calculated dosage of gonadotrophins in ovarian stimulation. The finding of a substantial longitudinal variation in AMH question one recording being sufficient in quantifying gonadotrophins for ovarian stimulation, decision making and prognostication related to infertility treatment and counseling. Occasionally, commercial assays may fail to recognize specific AMH cleavage-products.

KW - Anti mullerian hormone

KW - Antral follicle count

KW - Biomarkers

KW - Female infertility

KW - Infertility counseling

U2 - 10.3389/fendo.2018.00603

DO - 10.3389/fendo.2018.00603

M3 - Journal article

AN - SCOPUS:85055166334

VL - 9

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

SN - 1664-2392

IS - OCT

M1 - 595

ER -

ID: 218519996