TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells. / Zhang, J; Salojin, K; Gao, J X; Cameron, M; Geisler, C; Delovitch, T L.
In: Journal of Immunology, Vol. 161, No. 6, 1998, p. 2930-7.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells
AU - Zhang, J
AU - Salojin, K
AU - Gao, J X
AU - Cameron, M
AU - Geisler, C
AU - Delovitch, T L
N1 - Keywords: Animals; Antigens, CD3; Calcium-Binding Proteins; Calnexin; Cell Differentiation; Cell Membrane; Humans; Hybridomas; Immune Sera; Jurkat Cells; Lymphocyte Activation; Macromolecular Substances; Membrane Proteins; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocyte Subsets
PY - 1998
Y1 - 1998
N2 - The TCR is a multisubunit complex composed of the clonotypic alpha/beta disulfide-linked heterodimer and noncovalently linked invariant CD3 gamma epsilon and CD3 delta epsilon and TCR zeta chains. Recent studies demonstrate that the surface expression of CD3 components can occur independently of the clonotypic TCR complexes in both thymocytes and splenic T cells. In this study, we report that free noncovalently associated TCR alpha beta heterodimers that exist independently of CD3 and TCR zeta chains are expressed on the cell surface of immature thymocytes and peripheral T cells, but not of T cell lines and T cell hybridomas. This suggests that the regulation of surface expression of TCR alpha beta heterodimers differs between primary T cells and T cell lines or T cell hybridomas. The isolation and biochemical characterization of surface clonotype-independent CD3 complexes and free membrane-associated TCR alpha beta complexes may provide a structural basis for the quantitative difference in amount of T cell proliferation stimulated by anti-CD3 epsilon and anti-TCR beta.
AB - The TCR is a multisubunit complex composed of the clonotypic alpha/beta disulfide-linked heterodimer and noncovalently linked invariant CD3 gamma epsilon and CD3 delta epsilon and TCR zeta chains. Recent studies demonstrate that the surface expression of CD3 components can occur independently of the clonotypic TCR complexes in both thymocytes and splenic T cells. In this study, we report that free noncovalently associated TCR alpha beta heterodimers that exist independently of CD3 and TCR zeta chains are expressed on the cell surface of immature thymocytes and peripheral T cells, but not of T cell lines and T cell hybridomas. This suggests that the regulation of surface expression of TCR alpha beta heterodimers differs between primary T cells and T cell lines or T cell hybridomas. The isolation and biochemical characterization of surface clonotype-independent CD3 complexes and free membrane-associated TCR alpha beta complexes may provide a structural basis for the quantitative difference in amount of T cell proliferation stimulated by anti-CD3 epsilon and anti-TCR beta.
M3 - Journal article
C2 - 9743355
VL - 161
SP - 2930
EP - 2937
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -
ID: 8545338