Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
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Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats. / Andersen, Sofie Bech; Taghavi, Iman; Søgaard, Stinne Byrholdt; Hoyos, Carlos Armando Villagómez; Nielsen, Michael Bachmann; Jensen, Jørgen Arendt; Sørensen, Charlotte Mehlin.
In: Diagnostics, Vol. 12, No. 5, 1111, 05.2022, p. 1-15.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
AU - Andersen, Sofie Bech
AU - Taghavi, Iman
AU - Søgaard, Stinne Byrholdt
AU - Hoyos, Carlos Armando Villagómez
AU - Nielsen, Michael Bachmann
AU - Jensen, Jørgen Arendt
AU - Sørensen, Charlotte Mehlin
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5
Y1 - 2022/5
N2 - Super-resolution ultrasound imaging, based on the localization and tracking of single in-travascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclu-sively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.
AB - Super-resolution ultrasound imaging, based on the localization and tracking of single in-travascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclu-sively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.
KW - contrast-enhanced ultrasound
KW - kidneys
KW - microvascular flow
KW - prazosin
KW - Sprague Dawley rats
KW - ultrasound localization microscopy
UR - http://www.scopus.com/inward/record.url?scp=85129668213&partnerID=8YFLogxK
U2 - 10.3390/diagnostics12051111
DO - 10.3390/diagnostics12051111
M3 - Journal article
C2 - 35626267
AN - SCOPUS:85129668213
VL - 12
SP - 1
EP - 15
JO - Diagnostics
JF - Diagnostics
SN - 2075-4418
IS - 5
M1 - 1111
ER -
ID: 306450389