Sunflower (Helianthus annuus) fatty acid synthase complex: β-Ketoacyl-[acyl carrier protein] reductase genes
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Sunflower (Helianthus annuus) fatty acid synthase complex : β-Ketoacyl-[acyl carrier protein] reductase genes. / González-Thuillier, Irene; Venegas-Calerón, Mónica; Moreno-Pérez, Antonio J.; Salas, Joaquín J.; Garcés, Rafael; von Wettstein-Knowles, Penny; Martínez-Force, Enrique.
In: Plant Physiology and Biochemistry, Vol. 166, 2021, p. 689-699.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sunflower (Helianthus annuus) fatty acid synthase complex
T2 - β-Ketoacyl-[acyl carrier protein] reductase genes
AU - González-Thuillier, Irene
AU - Venegas-Calerón, Mónica
AU - Moreno-Pérez, Antonio J.
AU - Salas, Joaquín J.
AU - Garcés, Rafael
AU - von Wettstein-Knowles, Penny
AU - Martínez-Force, Enrique
N1 - Publisher Copyright: © 2021 The Authors
PY - 2021
Y1 - 2021
N2 - Fatty acids play many roles in plants, but the function of some key genes involved in fatty acid biosynthesis in plant development are not yet properly understood. Here, we clone two β-ketoacyl-[ACP] reductase (KAR) genes from sunflower, HaKAR1 and HaKAR2, and characterize their functional roles. The enzymes cloned were the only two copies present in the sunflower genome. Both displayed a high degree of similarity, but their promoters infer different regulation. The two sunflower KAR genes were constitutively expressed in all tissues examined, being maximum in developing cotyledons at the start of oil synthesis. Over-expression of HaKAR1 in E. coli changed the fatty acid composition by promoting the elongation of C16:0 to C18:0 fatty acids. The enzymatic characterization of HaKAR1 revealed similar kinetic parameters to homologues from other oil accumulating species. The results point to a partially functional redundancy between HaKAR1 and HaKAR2. This study clearly revealed that these genes play a prominent role in de novo fatty acids synthesis in sunflower seeds.
AB - Fatty acids play many roles in plants, but the function of some key genes involved in fatty acid biosynthesis in plant development are not yet properly understood. Here, we clone two β-ketoacyl-[ACP] reductase (KAR) genes from sunflower, HaKAR1 and HaKAR2, and characterize their functional roles. The enzymes cloned were the only two copies present in the sunflower genome. Both displayed a high degree of similarity, but their promoters infer different regulation. The two sunflower KAR genes were constitutively expressed in all tissues examined, being maximum in developing cotyledons at the start of oil synthesis. Over-expression of HaKAR1 in E. coli changed the fatty acid composition by promoting the elongation of C16:0 to C18:0 fatty acids. The enzymatic characterization of HaKAR1 revealed similar kinetic parameters to homologues from other oil accumulating species. The results point to a partially functional redundancy between HaKAR1 and HaKAR2. This study clearly revealed that these genes play a prominent role in de novo fatty acids synthesis in sunflower seeds.
KW - Fatty acid synthase (FAS)
KW - Helianthus annuus
KW - Oil biosynthesis
KW - Short-chain dehydrogenases/reductases (SDRs) family
KW - Substrate specificity
KW - WRINKLED1 (WRI1)
KW - β-ketoacyl-[ACP] reductase (KAR)
U2 - 10.1016/j.plaphy.2021.06.048
DO - 10.1016/j.plaphy.2021.06.048
M3 - Journal article
C2 - 34214779
AN - SCOPUS:85111019478
VL - 166
SP - 689
EP - 699
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
ER -
ID: 306894801