Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch
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Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch. / Møller, U.; Keiding, N.; Engel, F.
In: Cell and Tissue Kinetics, Vol. 15, No. 2, 03.1982, p. 157-168.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Subclassification of cells in S phase in a partially synchronized cell system.
T2 - Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch
AU - Møller, U.
AU - Keiding, N.
AU - Engel, F.
PY - 1982/3
Y1 - 1982/3
N2 - A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.
AB - A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.
UR - http://www.scopus.com/inward/record.url?scp=0020038983&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2184.1982.tb01034.x
DO - 10.1111/j.1365-2184.1982.tb01034.x
M3 - Journal article
C2 - 7066958
VL - 15
SP - 157
EP - 168
JO - Cell and Tissue Kinetics
JF - Cell and Tissue Kinetics
SN - 0008-8730
IS - 2
ER -
ID: 203011424