Stimulation of arachidonic acid metabolism in rat peritoneal macrophages by thapsigargin, a non-(12-O-tetradecanoylphorbol-13-acetate) (TPA)-type tumor promoter
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Stimulation of arachidonic acid metabolism in rat peritoneal macrophages by thapsigargin, a non-(12-O-tetradecanoylphorbol-13-acetate) (TPA)-type tumor promoter. / Ohuchi, Kazuo; Sugawara, Tadaki; Watanabe, Masako; Hirasawa, Noriyasu; Tsurufuji, Susumu; Fujiki, Hirota; Sugimura, Takashi; Christensen, S. B.
In: Journal of Cancer Research and Clinical Oncology, Vol. 113, No. 4, 01.06.1987, p. 319-324.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stimulation of arachidonic acid metabolism in rat peritoneal macrophages by thapsigargin, a non-(12-O-tetradecanoylphorbol-13-acetate) (TPA)-type tumor promoter
AU - Ohuchi, Kazuo
AU - Sugawara, Tadaki
AU - Watanabe, Masako
AU - Hirasawa, Noriyasu
AU - Tsurufuji, Susumu
AU - Fujiki, Hirota
AU - Sugimura, Takashi
AU - Christensen, S. B.
PY - 1987/6/1
Y1 - 1987/6/1
N2 - The effects of thapsigargin, which is a histamine secretagogue and has recently been found to be a non-(12-O-tetradecanoylphorbol-13-acetate) (TPA)-type tumor promoter in two-stage carcinogenesis using mouse skin, on arachidonic acid metabolism in rat peritoneal macrophages were examined. The release of radioactivity from 3H arachidonic acid-labeled macrophages was increased at doses more than 10 ng/ml. Prostaglandin E2 production was also increased dose-dependently without inducing prominent changes in cell morphology. The potency to stimulate prostaglandin E2 production by thapsigargin was stronger than that by TPA at a dose of 10 ng/ml when measured 6 h after the incubation. HPLC analysis revealed that thapsigargin stimulated the production of lipoxygenase products such as leukotriene B4 and 12-hydroxyeicosatetraenoic acid as well as cyclooxygenase products such as prostaglandin E2 and 6-ketoprostaglandin F1α. Thapsigargicin, an analogue of thapsigargin, also stimulated prostaglandin E2 production. The mechanism of the action of thapsigargin was discussed. It was confirmed that the tumor promoters are associated with the activity to stimulate arachidonic acid metabolism irrespective of their type, TPA-type or non-TPA-type.
AB - The effects of thapsigargin, which is a histamine secretagogue and has recently been found to be a non-(12-O-tetradecanoylphorbol-13-acetate) (TPA)-type tumor promoter in two-stage carcinogenesis using mouse skin, on arachidonic acid metabolism in rat peritoneal macrophages were examined. The release of radioactivity from 3H arachidonic acid-labeled macrophages was increased at doses more than 10 ng/ml. Prostaglandin E2 production was also increased dose-dependently without inducing prominent changes in cell morphology. The potency to stimulate prostaglandin E2 production by thapsigargin was stronger than that by TPA at a dose of 10 ng/ml when measured 6 h after the incubation. HPLC analysis revealed that thapsigargin stimulated the production of lipoxygenase products such as leukotriene B4 and 12-hydroxyeicosatetraenoic acid as well as cyclooxygenase products such as prostaglandin E2 and 6-ketoprostaglandin F1α. Thapsigargicin, an analogue of thapsigargin, also stimulated prostaglandin E2 production. The mechanism of the action of thapsigargin was discussed. It was confirmed that the tumor promoters are associated with the activity to stimulate arachidonic acid metabolism irrespective of their type, TPA-type or non-TPA-type.
KW - Archidonic acid
KW - Macrophages
KW - Non-TPA-type tumor promoter
KW - Prostaglandin E
KW - Thapsigargin
UR - http://www.scopus.com/inward/record.url?scp=0023219791&partnerID=8YFLogxK
U2 - 10.1007/BF00397715
DO - 10.1007/BF00397715
M3 - Journal article
C2 - 3110174
AN - SCOPUS:0023219791
VL - 113
SP - 319
EP - 324
JO - Zeitschrift fur Krebsforschung
JF - Zeitschrift fur Krebsforschung
SN - 0171-5216
IS - 4
ER -
ID: 232599610