Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization

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Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization. / Boye, M.; Jensen, T. K.; MØller, K.; Leser, T. D.; Jorsal, S. E.

In: Veterinary Pathology, Vol. 35, No. 2, 03.1998, p. 153-156.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boye, M, Jensen, TK, MØller, K, Leser, TD & Jorsal, SE 1998, 'Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization', Veterinary Pathology, vol. 35, no. 2, pp. 153-156. https://doi.org/10.1177/030098589803500212

APA

Boye, M., Jensen, T. K., MØller, K., Leser, T. D., & Jorsal, S. E. (1998). Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization. Veterinary Pathology, 35(2), 153-156. https://doi.org/10.1177/030098589803500212

Vancouver

Boye M, Jensen TK, MØller K, Leser TD, Jorsal SE. Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization. Veterinary Pathology. 1998 Mar;35(2):153-156. https://doi.org/10.1177/030098589803500212

Author

Boye, M. ; Jensen, T. K. ; MØller, K. ; Leser, T. D. ; Jorsal, S. E. / Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization. In: Veterinary Pathology. 1998 ; Vol. 35, No. 2. pp. 153-156.

Bibtex

@article{d6b30e035dac4727bdeb896d59aad431,
title = "Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization",
abstract = "Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.",
keywords = "In situ hybridization, Indirect immunofluorescence assay, Lawsonia intracellularis, Porcine proliferative enteropathy",
author = "M. Boye and Jensen, {T. K.} and K. M{\O}ller and Leser, {T. D.} and Jorsal, {S. E.}",
year = "1998",
month = mar,
doi = "10.1177/030098589803500212",
language = "English",
volume = "35",
pages = "153--156",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications",
number = "2",

}

RIS

TY - JOUR

T1 - Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization

AU - Boye, M.

AU - Jensen, T. K.

AU - MØller, K.

AU - Leser, T. D.

AU - Jorsal, S. E.

PY - 1998/3

Y1 - 1998/3

N2 - Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

AB - Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

KW - In situ hybridization

KW - Indirect immunofluorescence assay

KW - Lawsonia intracellularis

KW - Porcine proliferative enteropathy

UR - http://www.scopus.com/inward/record.url?scp=0032012985&partnerID=8YFLogxK

U2 - 10.1177/030098589803500212

DO - 10.1177/030098589803500212

M3 - Journal article

C2 - 9539372

AN - SCOPUS:0032012985

VL - 35

SP - 153

EP - 156

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 2

ER -

ID: 339896450