Site-Specific DC Surface Signatures Influence CD4+ T Cell Co-stimulation and Lung-Homing
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Site-Specific DC Surface Signatures Influence CD4+ T Cell Co-stimulation and Lung-Homing. / Pejoski, David; Ballester, Marie; Auderset, Floriane; Vono, Maria; Christensen, Dennis; Andersen, Peter; Lambert, Paul Henri; Siegrist, Claire Anne.
In: Frontiers in Immunology, Vol. 10, No. JULY, 1650, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Site-Specific DC Surface Signatures Influence CD4+ T Cell Co-stimulation and Lung-Homing
AU - Pejoski, David
AU - Ballester, Marie
AU - Auderset, Floriane
AU - Vono, Maria
AU - Christensen, Dennis
AU - Andersen, Peter
AU - Lambert, Paul Henri
AU - Siegrist, Claire Anne
PY - 2019
Y1 - 2019
N2 - Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble “imprinting” signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag+ DCs in the lung and muscle-draining LNs. Higher frequencies of Ag+ CD11b+ DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag+ CD11b+ MedLN DCs were qualitatively superior at priming CD4+ T cells, which then expressed CD49a+ CXCR3+ and preferentially trafficked into the lung parenchyma. CD11b+ DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag+ DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4+ T cells.
AB - Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble “imprinting” signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag+ DCs in the lung and muscle-draining LNs. Higher frequencies of Ag+ CD11b+ DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag+ CD11b+ MedLN DCs were qualitatively superior at priming CD4+ T cells, which then expressed CD49a+ CXCR3+ and preferentially trafficked into the lung parenchyma. CD11b+ DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag+ DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4+ T cells.
KW - CD11b+ dendritic cells
KW - Costimulation
KW - Lung CD4+ T cells
KW - Lung homing
KW - Tissue imprinting
KW - Vaccination route
UR - https://www.frontiersin.org/articles/10.3389/fimmu.2019.02640/full
U2 - 10.3389/fimmu.2019.01650
DO - 10.3389/fimmu.2019.01650
M3 - Journal article
C2 - 31396211
AN - SCOPUS:85069493984
VL - 10
JO - Frontiers in Immunology
JF - Frontiers in Immunology
SN - 1664-3224
IS - JULY
M1 - 1650
ER -
ID: 226877420