Single-cell tracking reveals super-spreading brain cancer cells with high persistence
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Single-cell tracking reveals super-spreading brain cancer cells with high persistence. / Nousi, Aimilia; Sogaard, Maria Tangen; Audion, Melanie; Jauffred, Liselotte.
In: Biochemistry and Biophysics Reports, Vol. 28, 101120, 01.12.2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Single-cell tracking reveals super-spreading brain cancer cells with high persistence
AU - Nousi, Aimilia
AU - Sogaard, Maria Tangen
AU - Audion, Melanie
AU - Jauffred, Liselotte
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Cell migration is a fundamental characteristic of vital processes such as tissue morphogenesis, wound healing and immune cell homing to lymph nodes and inflamed or infected sites. Therefore, various brain defect diseases, chronic inflammatory diseases as well as tumor formation and metastasis are associated with aberrant or absent cell migration. We embedded multicellular brain cancer spheroids in Matrigel (TM) and utilized single-particle tracking to extract the paths of cells migrating away from the spheroids. We found that - in contrast to local invasion - single cell migration is independent of Matrigel (TM) concentration and is characterized by high directionality and persistence. Furthermore, we identified a subpopulation of super-spreading cells with >200-fold longer persistence times than the majority of cells. These results highlight yet another aspect of cell heterogeneity in tumors.
AB - Cell migration is a fundamental characteristic of vital processes such as tissue morphogenesis, wound healing and immune cell homing to lymph nodes and inflamed or infected sites. Therefore, various brain defect diseases, chronic inflammatory diseases as well as tumor formation and metastasis are associated with aberrant or absent cell migration. We embedded multicellular brain cancer spheroids in Matrigel (TM) and utilized single-particle tracking to extract the paths of cells migrating away from the spheroids. We found that - in contrast to local invasion - single cell migration is independent of Matrigel (TM) concentration and is characterized by high directionality and persistence. Furthermore, we identified a subpopulation of super-spreading cells with >200-fold longer persistence times than the majority of cells. These results highlight yet another aspect of cell heterogeneity in tumors.
KW - Invasion assay
KW - Glioblastoma
KW - Single-cell tracking
KW - Diffusion models
KW - BASEMENT-MEMBRANE MATRIX
KW - RANDOM MOTION
KW - ANOMALOUS DIFFUSION
KW - STOCHASTIC-MODEL
KW - TUMOR SPHEROIDS
KW - MIGRATION
KW - MOTILITY
KW - TRANSITION
KW - DYNAMICS
KW - GROWTH
U2 - 10.1016/j.bbrep.2021.101120
DO - 10.1016/j.bbrep.2021.101120
M3 - Journal article
C2 - 34541340
VL - 28
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
SN - 2405-5808
M1 - 101120
ER -
ID: 289234640