Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. / Lopez-Perez, Mary.
Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 79-90 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants
AU - Lopez-Perez, Mary
N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for that marker. After the separation, the viable homogeneous population can be used to grow in culture or for molecular analysis.
AB - Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for that marker. After the separation, the viable homogeneous population can be used to grow in culture or for molecular analysis.
KW - Animals
KW - Erythrocytes/metabolism
KW - Humans
KW - Malaria, Falciparum/parasitology
KW - Parasites/metabolism
KW - Plasmodium falciparum/metabolism
KW - Protozoan Proteins/metabolism
U2 - 10.1007/978-1-0716-2189-9_8
DO - 10.1007/978-1-0716-2189-9_8
M3 - Book chapter
C2 - 35881340
VL - 2470
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 79
EP - 90
BT - Malaria Immunology
PB - Humana Press
ER -
ID: 320649585