Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND: Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls.
METHODS: All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index.
RESULTS: Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease.
CONCLUSIONS: Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.
Original language | English |
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Journal | Scandinavian Journal of Gastroenterology |
Volume | 47 |
Issue number | 2 |
Pages (from-to) | 155-61 |
Number of pages | 7 |
ISSN | 0036-5521 |
DOIs | |
Publication status | Published - Feb 2012 |
- Acute Disease, Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Bacterial/blood, Bacterial Outer Membrane Proteins/immunology, Child, Chronic Disease, Colitis, Ulcerative/blood, Colonic Pouches/pathology, Crohn Disease/blood, Diarrhea/blood, Dysentery/blood, Escherichia coli/immunology, Escherichia coli Infections/complications, Escherichia coli Proteins/immunology, Female, Gastroenteritis/blood, Humans, Male, Middle Aged, Porins/immunology, Pouchitis/blood, Sensitivity and Specificity, Serologic Tests, Young Adult
Research areas
ID: 209679364