TY - JOUR

T1 - Separation of neutral compounds by microemulsion electrokinetic chromatography

T2 - fundamental studies on selectivity

AU - Gabel-Jensen, Charlotte

AU - Hansen, Steen Honore'

AU - Pedersen-Bjergaard, S

PY - 2001

Y1 - 2001

N2 - The selectivity of microemulsion electrokinetic chromatography (MEEKC) was studied utilizing some uncharged model compounds like aromatic amides, steroids, and esters of nicotinic acid. The cosurfactant of the microemulsion was found to be the most important factor affecting the selectivity, and alteration between 6.6% of 1-propanol, 1-butanol, tetrahydrofuran, and 2-ethoxyethanol caused several substantial changes in the migration order. In addition, the nature of the surfactant was found to significantly affect the selectivity. In this case, changes in order of migration was observed by replacement of half the content of sodium dodecyl sulfate (SDS) with either sodium dioctyl sulfosuccinate (SDOSS), 3-(N,N-dimethylmyristylammonio) propanesulfonate (MAPS), polyoxyethylene sorbitan monolaurate (Tween 21), and polyoxyethylene 23 lauryl ether (Brij 35). MEEKC was also accomplished with 3.3% of the anionic surfactant sodium cholate and with the cationic surfactant N-cetyl-N,N,N-trimethylammonium bromide (CTMA). Both provided substantial differences in selectivity as compared to the SDS-based systems. With SDS as surfacant, the concentration was varied within 1.0-4.5%. Minor selectivity changes were observed as the concentration of the surfacant was reduced, but the major effect was a reduction in the total migration time. The organic solvent of the microemulsion droplets was found only to have minor impact on the selectivity.

AB - The selectivity of microemulsion electrokinetic chromatography (MEEKC) was studied utilizing some uncharged model compounds like aromatic amides, steroids, and esters of nicotinic acid. The cosurfactant of the microemulsion was found to be the most important factor affecting the selectivity, and alteration between 6.6% of 1-propanol, 1-butanol, tetrahydrofuran, and 2-ethoxyethanol caused several substantial changes in the migration order. In addition, the nature of the surfactant was found to significantly affect the selectivity. In this case, changes in order of migration was observed by replacement of half the content of sodium dodecyl sulfate (SDS) with either sodium dioctyl sulfosuccinate (SDOSS), 3-(N,N-dimethylmyristylammonio) propanesulfonate (MAPS), polyoxyethylene sorbitan monolaurate (Tween 21), and polyoxyethylene 23 lauryl ether (Brij 35). MEEKC was also accomplished with 3.3% of the anionic surfactant sodium cholate and with the cationic surfactant N-cetyl-N,N,N-trimethylammonium bromide (CTMA). Both provided substantial differences in selectivity as compared to the SDS-based systems. With SDS as surfacant, the concentration was varied within 1.0-4.5%. Minor selectivity changes were observed as the concentration of the surfacant was reduced, but the major effect was a reduction in the total migration time. The organic solvent of the microemulsion droplets was found only to have minor impact on the selectivity.

KW - Chromatography

KW - Electrophoresis, Capillary

KW - Sensitivity and Specificity

U2 - 10.1002/1522-2683(200105)22:7<1330::AID-ELPS1330>3.0.CO;2-D

DO - 10.1002/1522-2683(200105)22:7<1330::AID-ELPS1330>3.0.CO;2-D

M3 - Journal article

C2 - 11379955

VL - 22

SP - 1330

EP - 1336

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 7

ER -