Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers: Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains
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Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers : Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains. / Saidenberg, Andre Becker Simoes; Franco, Leticia Soares; Reple, Jessica Nacarato; Hounmanou, Yaovi Mahuton Gildas; Casas, Monique Ribeiro Tiba; Cardoso, Brenda; Esposito, Fernanda; Lincopan, Nilton; Dalsgaard, Anders; Stegger, Marc; Knöbl, Terezinha.
In: Environmental Microbiology Reports, Vol. 15, No. 2, 2023, p. 119-128.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers
T2 - Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains
AU - Saidenberg, Andre Becker Simoes
AU - Franco, Leticia Soares
AU - Reple, Jessica Nacarato
AU - Hounmanou, Yaovi Mahuton Gildas
AU - Casas, Monique Ribeiro Tiba
AU - Cardoso, Brenda
AU - Esposito, Fernanda
AU - Lincopan, Nilton
AU - Dalsgaard, Anders
AU - Stegger, Marc
AU - Knöbl, Terezinha
N1 - © 2022 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.
PY - 2023
Y1 - 2023
N2 - Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (blaCMY-2 ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons. IncC carrying blaCTX-M-2 was only detected among SH (3/10). Mutation in the gyrA/parC genes was present in all SH, whereas SM harboured parC mutation plus qnrB19 on ColRNAI plasmids (3/4). In silico resistance overall corroborated with phenotypic results. Core genome phylogenies showed close clustering and high similarities between the Brazilian and poultry meat/food isolates from Europe, and to human isolates from European countries with documented import of Brazilian poultry meat. Conjugation assays with SM successfully transferred blaCMY-2 , and qnrB19 to an Escherichia coli recipient. The findings reinforce the ongoing antimicrobial resistance acquisition of SH and Minnesota and the risks for disseminating resistant strains and/or mobile elements which may increasingly affect importing countries and the need for controlling AMR in major poultry-exporting countries like Brazil.
AB - Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (blaCMY-2 ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons. IncC carrying blaCTX-M-2 was only detected among SH (3/10). Mutation in the gyrA/parC genes was present in all SH, whereas SM harboured parC mutation plus qnrB19 on ColRNAI plasmids (3/4). In silico resistance overall corroborated with phenotypic results. Core genome phylogenies showed close clustering and high similarities between the Brazilian and poultry meat/food isolates from Europe, and to human isolates from European countries with documented import of Brazilian poultry meat. Conjugation assays with SM successfully transferred blaCMY-2 , and qnrB19 to an Escherichia coli recipient. The findings reinforce the ongoing antimicrobial resistance acquisition of SH and Minnesota and the risks for disseminating resistant strains and/or mobile elements which may increasingly affect importing countries and the need for controlling AMR in major poultry-exporting countries like Brazil.
U2 - 10.1111/1758-2229.13132
DO - 10.1111/1758-2229.13132
M3 - Journal article
C2 - 36629129
VL - 15
SP - 119
EP - 128
JO - Environmental Microbiology Reports
JF - Environmental Microbiology Reports
SN - 1758-2229
IS - 2
ER -
ID: 333184228