Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection
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Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection. / Dahly, Elizabeth M; Gillingham, Melanie B; Guo, Ziwen; Murali, Sangita G; Nelson, David W; Holst, Jens Juul; Ney, Denise M.
In: American Journal of Physiology: Gastrointestinal and Liver Physiology, Vol. 284, No. 4, 04.2003, p. G670-82.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection
AU - Dahly, Elizabeth M
AU - Gillingham, Melanie B
AU - Guo, Ziwen
AU - Murali, Sangita G
AU - Nelson, David W
AU - Holst, Jens Juul
AU - Ney, Denise M
PY - 2003/4
Y1 - 2003/4
N2 - To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.
AB - To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.
KW - Adaptation, Physiological
KW - Animals
KW - Apoptosis
KW - Body Weight
KW - Cell Division
KW - Eating
KW - Enterocytes
KW - Glucagon
KW - Glucagon-Like Peptide 2
KW - Glucagon-Like Peptides
KW - Ileum
KW - Jejunum
KW - Male
KW - Parenteral Nutrition
KW - Peptides
KW - Proglucagon
KW - Protein Precursors
KW - Rats
KW - Rats, Sprague-Dawley
KW - Sucrase
U2 - 10.1152/ajpgi.00293.2002
DO - 10.1152/ajpgi.00293.2002
M3 - Journal article
C2 - 12505881
VL - 284
SP - G670-82
JO - American Journal of Physiology: Gastrointestinal and Liver Physiology
JF - American Journal of Physiology: Gastrointestinal and Liver Physiology
SN - 0193-1857
IS - 4
ER -
ID: 132056367