Recent advances in the measurement of adiponectin isoform distribution
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Recent advances in the measurement of adiponectin isoform distribution. / Magkos, Faidon; Sidossis, Labros S.
In: Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 10, No. 5, 2007, p. 571-575.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Recent advances in the measurement of adiponectin isoform distribution
AU - Magkos, Faidon
AU - Sidossis, Labros S
N1 - (Ekstern)
PY - 2007
Y1 - 2007
N2 - Purpose of review: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution.Recent findings: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases.Summary: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.
AB - Purpose of review: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution.Recent findings: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases.Summary: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.
KW - Adiponectin/chemistry
KW - Antibodies, Monoclonal/immunology
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - Molecular Structure
KW - Molecular Weight
KW - Multiprotein Complexes
KW - Protein Isoforms
U2 - 10.1097/MCO.0b013e3282bf6ea8
DO - 10.1097/MCO.0b013e3282bf6ea8
M3 - Review
C2 - 17693739
VL - 10
SP - 571
EP - 575
JO - Current Opinion in Clinical Nutrition and Metabolic Care
JF - Current Opinion in Clinical Nutrition and Metabolic Care
SN - 1363-1950
IS - 5
ER -
ID: 297124667