Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen
Research output: Contribution to journal › Journal article › Research › peer-review
Melanoma antigen recognized by T cell 1 (MART-1) is regarded as a candidate peptide for vaccination against malignant melanoma, and it is of importance to develop strategies to improve the vaccine-elicited T-cell activation towards MART-1. T-cell activation is, among other determinants, dependent on the density of specific major histocompatibility complex-peptide complexes on the surface of the antigen-presenting cell. In this study, we explored the cell-surface presentation of a substituted MART-1 peptide encoded by transfected minigenes. We investigated the potential of proteasomal targeting compared to non-proteasomal targeting of the epitope to increase its cell-surface presentation. Furthermore, we explored the potential of incorporating multiple minigenes instead of one to increase cell-surface presentation. We show that both proteasomal targeting and repetition of the minigene increase cell-surface presentation of the epitope and propose both these approaches as potential strategies in DNA vaccines to increase MART-1-specific T-cell activation.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 59 |
Issue number | 2 |
Pages (from-to) | 220-7 |
Number of pages | 7 |
ISSN | 0300-9475 |
Publication status | Published - 2004 |
Bibliographical note
Keywords: Amino Acid Sequence; Antigen Presentation; Antigens, Neoplasm; Blotting, Western; Cancer Vaccines; Cell Line; Cysteine Endopeptidases; Cytotoxicity Tests, Immunologic; Epitopes; Humans; Melanoma; Molecular Sequence Data; Multienzyme Complexes; Neoplasm Proteins; Plasmids; Polymerase Chain Reaction; Proteasome Endopeptidase Complex; Repetitive Sequences, Nucleic Acid; T-Lymphocytes; Transfection; Vaccines, DNA
ID: 8544376