Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Progastrin processing differs in 7B2 and PC2 knockout animals : A role for 7B2 independent of action on PC2. / Rehfeld, Jens F.; Lindberg, Iris; Friis-Hansen, Lennart.

In: FEBS Letters, Vol. 510, No. 1-2, 02.01.2002, p. 89-93.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rehfeld, JF, Lindberg, I & Friis-Hansen, L 2002, 'Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2', FEBS Letters, vol. 510, no. 1-2, pp. 89-93. https://doi.org/10.1016/S0014-5793(01)03234-3

APA

Rehfeld, J. F., Lindberg, I., & Friis-Hansen, L. (2002). Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2. FEBS Letters, 510(1-2), 89-93. https://doi.org/10.1016/S0014-5793(01)03234-3

Vancouver

Rehfeld JF, Lindberg I, Friis-Hansen L. Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2. FEBS Letters. 2002 Jan 2;510(1-2):89-93. https://doi.org/10.1016/S0014-5793(01)03234-3

Author

Rehfeld, Jens F. ; Lindberg, Iris ; Friis-Hansen, Lennart. / Progastrin processing differs in 7B2 and PC2 knockout animals : A role for 7B2 independent of action on PC2. In: FEBS Letters. 2002 ; Vol. 510, No. 1-2. pp. 89-93.

Bibtex

@article{daa2e104ae984c2e84b9b261166dea60,
title = "Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2",
abstract = "Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K53-K54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.",
keywords = "7B2, Chaperone, Gastrin, Progastrin, Prohormone convertase, Prohormone processing",
author = "Rehfeld, {Jens F.} and Iris Lindberg and Lennart Friis-Hansen",
note = "Funding Information: The skillful technical assistance of Rikke Gr{\o}nholt and Alice von der Lieth is gratefully acknowledged. The study was supported by grants from the Danish Medical Research Council, the Danish Biotechnology Program for Peptide Research and Cellular Communication and NIH Grants (DA05084 and DK49703).",
year = "2002",
month = jan,
day = "2",
doi = "10.1016/S0014-5793(01)03234-3",
language = "English",
volume = "510",
pages = "89--93",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1-2",

}

RIS

TY - JOUR

T1 - Progastrin processing differs in 7B2 and PC2 knockout animals

T2 - A role for 7B2 independent of action on PC2

AU - Rehfeld, Jens F.

AU - Lindberg, Iris

AU - Friis-Hansen, Lennart

N1 - Funding Information: The skillful technical assistance of Rikke Grønholt and Alice von der Lieth is gratefully acknowledged. The study was supported by grants from the Danish Medical Research Council, the Danish Biotechnology Program for Peptide Research and Cellular Communication and NIH Grants (DA05084 and DK49703).

PY - 2002/1/2

Y1 - 2002/1/2

N2 - Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K53-K54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.

AB - Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K53-K54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.

KW - 7B2

KW - Chaperone

KW - Gastrin

KW - Progastrin

KW - Prohormone convertase

KW - Prohormone processing

UR - http://www.scopus.com/inward/record.url?scp=0037005995&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(01)03234-3

DO - 10.1016/S0014-5793(01)03234-3

M3 - Journal article

C2 - 11755537

AN - SCOPUS:0037005995

VL - 510

SP - 89

EP - 93

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1-2

ER -

ID: 310766240