Prevalence, antimicrobial resistance, and molecular characterization of methicillin-resistant Staphylococcus aureus from bulk tank milk of dairy herds
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Prevalence, antimicrobial resistance, and molecular characterization of methicillin-resistant Staphylococcus aureus from bulk tank milk of dairy herds. / Kreausukon, K.; Fetsch, A.; Kraushaar, B.; Alt, K.; Müller, K.; Krömker, V.; Zessin, K. H.; Käsbohrer, A.; Tenhagen, B. A.
In: Journal of Dairy Science, Vol. 95, No. 8, 01.08.2012, p. 4382-4388.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Prevalence, antimicrobial resistance, and molecular characterization of methicillin-resistant Staphylococcus aureus from bulk tank milk of dairy herds
AU - Kreausukon, K.
AU - Fetsch, A.
AU - Kraushaar, B.
AU - Alt, K.
AU - Müller, K.
AU - Krömker, V.
AU - Zessin, K. H.
AU - Käsbohrer, A.
AU - Tenhagen, B. A.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - It was the objective of the study to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in bulk tank milk from German dairy herds and to characterize isolates from bulk tank milk with respect to their Staph. aureus protein A (spa) and staphylococcal cassette chromosome mec (SCC. mec) type, their phenotypic antimicrobial resistance and resistance- resp. virulence-associated genes using broth microdilution and a microarray for Staph. aureus. Bulk tank milk samples (25. mL) were tested for MRSA using a 2-step selective enrichment protocol. Presumptive MRSA were confirmed by PCR. Thirty-six isolates collected from bulk tank milk of dairy herds in 2009 and 2010 were included in the characterization. All isolates displayed spa-types assigned to the clonal complex CC398. Based on the epidemiological cut-off values for the interpretation of minimum inhibitory concentrations isolates were resistant to tetracycline (100%), clindamycin (58%), erythromycin (52%), quinupristin/dalfopristin (36%), and kanamycin (27%). Isolates did not carry genes associated with typical virulence factors for Staph. aureus such as the Panton-Valentine leukocidin. However, they did carry hemolysin genes. Livestock-associated MRSA of CC398 does occur in German dairy herds and the strains have similar properties as described for strains from pigs.
AB - It was the objective of the study to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in bulk tank milk from German dairy herds and to characterize isolates from bulk tank milk with respect to their Staph. aureus protein A (spa) and staphylococcal cassette chromosome mec (SCC. mec) type, their phenotypic antimicrobial resistance and resistance- resp. virulence-associated genes using broth microdilution and a microarray for Staph. aureus. Bulk tank milk samples (25. mL) were tested for MRSA using a 2-step selective enrichment protocol. Presumptive MRSA were confirmed by PCR. Thirty-six isolates collected from bulk tank milk of dairy herds in 2009 and 2010 were included in the characterization. All isolates displayed spa-types assigned to the clonal complex CC398. Based on the epidemiological cut-off values for the interpretation of minimum inhibitory concentrations isolates were resistant to tetracycline (100%), clindamycin (58%), erythromycin (52%), quinupristin/dalfopristin (36%), and kanamycin (27%). Isolates did not carry genes associated with typical virulence factors for Staph. aureus such as the Panton-Valentine leukocidin. However, they did carry hemolysin genes. Livestock-associated MRSA of CC398 does occur in German dairy herds and the strains have similar properties as described for strains from pigs.
KW - Antimicrobial resistance
KW - Dairy cow
KW - Methicillin
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=84864071885&partnerID=8YFLogxK
U2 - 10.3168/jds.2011-5198
DO - 10.3168/jds.2011-5198
M3 - Journal article
C2 - 22818451
AN - SCOPUS:84864071885
VL - 95
SP - 4382
EP - 4388
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 8
ER -
ID: 237097308