PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Antisense PNA-peptide conjugates targeting essential bacterial genes have shown interesting potential for discovery of novel precision antibiotics. In this context, the minimal inhibitory concentration (MIC) assay is used to assess and compare the antimicrobial activity of natural as well as synthetic antimicrobial compounds. Here, we describe the determination of the minimal inhibitory concentration of peptide-PNA conjugates against Escherichia coli. This method can be expanded to include minimal bactericidal concentration (MBC) determination and kill-curve kinetics.
Original language | English |
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Title of host publication | Peptide Nucleic Acids |
Number of pages | 9 |
Publisher | Humana Press |
Publication date | 2020 |
Pages | 231-239 |
ISBN (Print) | 978-1-0716-0242-3 |
ISBN (Electronic) | 978-1-0716-0243-0 |
DOIs | |
Publication status | Published - 2020 |
Series | Methods in Molecular Biology |
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Volume | 2105 |
ISSN | 1064-3745 |
- Antimicrobials, Antisense, Minimal bactericidal concentration (MBC), Minimal inhibitory concentration (MIC), Translational inhibition
Research areas
ID: 239812223