PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

PNA Antisense Targeting in Bacteria : Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates. / Goltermann, Lise; Nielsen, Peter E.

Peptide Nucleic Acids. Humana Press, 2020. p. 231-239 (Methods in Molecular Biology, Vol. 2105).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Goltermann, L & Nielsen, PE 2020, PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates. in Peptide Nucleic Acids. Humana Press, Methods in Molecular Biology, vol. 2105, pp. 231-239. https://doi.org/10.1007/978-1-0716-0243-0_14

APA

Goltermann, L., & Nielsen, P. E. (2020). PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates. In Peptide Nucleic Acids (pp. 231-239). Humana Press. Methods in Molecular Biology Vol. 2105 https://doi.org/10.1007/978-1-0716-0243-0_14

Vancouver

Goltermann L, Nielsen PE. PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates. In Peptide Nucleic Acids. Humana Press. 2020. p. 231-239. (Methods in Molecular Biology, Vol. 2105). https://doi.org/10.1007/978-1-0716-0243-0_14

Author

Goltermann, Lise ; Nielsen, Peter E. / PNA Antisense Targeting in Bacteria : Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates. Peptide Nucleic Acids. Humana Press, 2020. pp. 231-239 (Methods in Molecular Biology, Vol. 2105).

Bibtex

@inbook{2097254d3551472aa8626bb9014cb67f,
title = "PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates",
abstract = "Antisense PNA-peptide conjugates targeting essential bacterial genes have shown interesting potential for discovery of novel precision antibiotics. In this context, the minimal inhibitory concentration (MIC) assay is used to assess and compare the antimicrobial activity of natural as well as synthetic antimicrobial compounds. Here, we describe the determination of the minimal inhibitory concentration of peptide-PNA conjugates against Escherichia coli. This method can be expanded to include minimal bactericidal concentration (MBC) determination and kill-curve kinetics.",
keywords = "Antimicrobials, Antisense, Minimal bactericidal concentration (MBC), Minimal inhibitory concentration (MIC), Translational inhibition",
author = "Lise Goltermann and Nielsen, {Peter E.}",
year = "2020",
doi = "10.1007/978-1-0716-0243-0_14",
language = "English",
isbn = "978-1-0716-0242-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "231--239",
booktitle = "Peptide Nucleic Acids",
address = "United States",

}

RIS

TY - CHAP

T1 - PNA Antisense Targeting in Bacteria

T2 - Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates

AU - Goltermann, Lise

AU - Nielsen, Peter E.

PY - 2020

Y1 - 2020

N2 - Antisense PNA-peptide conjugates targeting essential bacterial genes have shown interesting potential for discovery of novel precision antibiotics. In this context, the minimal inhibitory concentration (MIC) assay is used to assess and compare the antimicrobial activity of natural as well as synthetic antimicrobial compounds. Here, we describe the determination of the minimal inhibitory concentration of peptide-PNA conjugates against Escherichia coli. This method can be expanded to include minimal bactericidal concentration (MBC) determination and kill-curve kinetics.

AB - Antisense PNA-peptide conjugates targeting essential bacterial genes have shown interesting potential for discovery of novel precision antibiotics. In this context, the minimal inhibitory concentration (MIC) assay is used to assess and compare the antimicrobial activity of natural as well as synthetic antimicrobial compounds. Here, we describe the determination of the minimal inhibitory concentration of peptide-PNA conjugates against Escherichia coli. This method can be expanded to include minimal bactericidal concentration (MBC) determination and kill-curve kinetics.

KW - Antimicrobials

KW - Antisense

KW - Minimal bactericidal concentration (MBC)

KW - Minimal inhibitory concentration (MIC)

KW - Translational inhibition

U2 - 10.1007/978-1-0716-0243-0_14

DO - 10.1007/978-1-0716-0243-0_14

M3 - Book chapter

C2 - 32088874

AN - SCOPUS:85079916627

SN - 978-1-0716-0242-3

T3 - Methods in Molecular Biology

SP - 231

EP - 239

BT - Peptide Nucleic Acids

PB - Humana Press

ER -

ID: 239812223