Plasma homocysteine concentrations in Greek children are influenced by an interaction between the methylenetetrahydrofolate reductase C677T genotype and folate status
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Plasma homocysteine concentrations in Greek children are influenced by an interaction between the methylenetetrahydrofolate reductase C677T genotype and folate status. / Papoutsakis, Constantina; Yiannakouris, Nikos; Manios, Yannis; Papaconstantinou, Evaggelos; Magkos, Faidon; Schulpis, Kleopatra H; Zampelas, Antonis; Matalas, Antonia L.
In: Journal of Nutrition, Vol. 135, No. 3, 2005, p. 383-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plasma homocysteine concentrations in Greek children are influenced by an interaction between the methylenetetrahydrofolate reductase C677T genotype and folate status
AU - Papoutsakis, Constantina
AU - Yiannakouris, Nikos
AU - Manios, Yannis
AU - Papaconstantinou, Evaggelos
AU - Magkos, Faidon
AU - Schulpis, Kleopatra H
AU - Zampelas, Antonis
AU - Matalas, Antonia L
N1 - (Ekstern)
PY - 2005
Y1 - 2005
N2 - Risk factors established at young ages may set the stage for later cardiovascular disease (CVD). Elevated total homocysteine (tHcy) in blood is an emerging risk factor for CVD, yet few studies have been conducted in children, especially in the Mediterranean. We described plasma tHcy concentrations in a group of healthy Greek children and examined its relation with physiologic, metabolic, and genetic variables. Fasting blood samples were collected from 186 students, 11.6 +/- 0.4 years old, and tHcy, folate, vitamin B-12, and routine biochemistry variables in plasma were measured. The methylenetetrahydrolate reductase (MTHFR) C677T genotype was determined and anthropometric and dietary data were obtained. The distribution of tHcy was positively skewed with a median of 7.9 micromol/L (mean: 8.2 +/- 2.3 micromol/L; range: 4.4-22.2 micromol/L). tHcy was inversely related to plasma folate (r = -0.34, P < 0.0001), vitamin B-12 (r = -0.20, P = 0.008), and glucose (r = -0.15, P = 0.045). An interaction between the MTHFR genotype and plasma folate on tHcy was detected (P = 0.047). Specifically, the homozygous mutant TT genotype was associated with higher tHcy only in children with lower plasma folate (< 19.9 nmol/L), (P = 0.012). In our sample of healthy Greek children, plasma tHcy concentrations were higher than values reported in children of Northern European descent and were associated with folate, vitamin B-12, and glucose in plasma. The results also show that, similar to adults, plasma folate concentration is important in determining the contribution of the MTHFR C677T mutation to tHcy concentrations in children.
AB - Risk factors established at young ages may set the stage for later cardiovascular disease (CVD). Elevated total homocysteine (tHcy) in blood is an emerging risk factor for CVD, yet few studies have been conducted in children, especially in the Mediterranean. We described plasma tHcy concentrations in a group of healthy Greek children and examined its relation with physiologic, metabolic, and genetic variables. Fasting blood samples were collected from 186 students, 11.6 +/- 0.4 years old, and tHcy, folate, vitamin B-12, and routine biochemistry variables in plasma were measured. The methylenetetrahydrolate reductase (MTHFR) C677T genotype was determined and anthropometric and dietary data were obtained. The distribution of tHcy was positively skewed with a median of 7.9 micromol/L (mean: 8.2 +/- 2.3 micromol/L; range: 4.4-22.2 micromol/L). tHcy was inversely related to plasma folate (r = -0.34, P < 0.0001), vitamin B-12 (r = -0.20, P = 0.008), and glucose (r = -0.15, P = 0.045). An interaction between the MTHFR genotype and plasma folate on tHcy was detected (P = 0.047). Specifically, the homozygous mutant TT genotype was associated with higher tHcy only in children with lower plasma folate (< 19.9 nmol/L), (P = 0.012). In our sample of healthy Greek children, plasma tHcy concentrations were higher than values reported in children of Northern European descent and were associated with folate, vitamin B-12, and glucose in plasma. The results also show that, similar to adults, plasma folate concentration is important in determining the contribution of the MTHFR C677T mutation to tHcy concentrations in children.
KW - Biomarkers/blood
KW - Child
KW - Coronary Disease/epidemiology
KW - Female
KW - Folic Acid/blood
KW - Genotype
KW - Greece
KW - Homocysteine/blood
KW - Humans
KW - Male
KW - Methylenetetrahydrofolate Reductase (NADPH2)/genetics
KW - Polymorphism, Single Nucleotide
KW - Risk Factors
U2 - 10.1093/jn/135.3.383
DO - 10.1093/jn/135.3.383
M3 - Journal article
C2 - 15735067
VL - 135
SP - 383
EP - 388
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 3
ER -
ID: 297236775