Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM)

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Standard

Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM). / Mølbak, Lars; Schou, Kirstine Klitgaard; Jensen, Tim Kåre; Fossi, M.; Boye, Mette.

2006.

Research output: Contribution to conference › Conference abstract for conference › Research › peer-review

Harvard

Mølbak, L, Schou, KK, Jensen, TK, Fossi, M & Boye, M 2006, 'Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM)'.

APA

Mølbak, L., Schou, K. K., Jensen, T. K., Fossi, M., & Boye, M. (2006). Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM).

Vancouver

Mølbak L, Schou KK, Jensen TK, Fossi M, Boye M. Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM). 2006.

Author

Mølbak, Lars ; Schou, Kirstine Klitgaard ; Jensen, Tim Kåre ; Fossi, M. ; Boye, Mette. / Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM).

Bibtex

@conference{2598354e91a14c11a42c6047861bc472,
title = "Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM)",
abstract = "Helical-shaped bacteria resembling Spirochaetes commonly are present in the gastrointestinal tract of animals and humans. Culturing of Spirochaetes is in general fastidious and not always successful. Here, a new DNA isolation approach for prokaryotic cells in formalin-fixed paraffin-embedded tissue samples is applied for the identification of an unknown Spirochaete. The tissue samples from colon were obtained from a previous study (1). Sectioning, in situ hybridization, LCM, DNA extraction, PCR reaction and sequencing were done according to Klitgaard et al. (2). The unknown invasive Spirochaetes species was recognized in pigs by using fluorescent in situ hybridization, followed by laser capture microdissection (LCM) of the targeted cells from the colonic mucosa. Direct 16S rRNA gene PCR was performed from the dissected micro-colonies. A phylogenetic analysis clustered the 16S rRNA gene in the Treponema genus group. The closest related Treponema type strain was T. bryantii (",
author = "Lars M{\o}lbak and Schou, {Kirstine Klitgaard} and Jensen, {Tim K{\aa}re} and M. Fossi and Mette Boye",
year = "2006",
language = "English",

}

RIS

TY - ABST

T1 - Phylogeny and distribution of an unknown Treponema sp. associated with porcine colitis by using in situ hybridization and laser capture microdissection (LCM)

AU - Mølbak, Lars

AU - Schou, Kirstine Klitgaard

AU - Jensen, Tim Kåre

AU - Fossi, M.

AU - Boye, Mette

PY - 2006

Y1 - 2006

N2 - Helical-shaped bacteria resembling Spirochaetes commonly are present in the gastrointestinal tract of animals and humans. Culturing of Spirochaetes is in general fastidious and not always successful. Here, a new DNA isolation approach for prokaryotic cells in formalin-fixed paraffin-embedded tissue samples is applied for the identification of an unknown Spirochaete. The tissue samples from colon were obtained from a previous study (1). Sectioning, in situ hybridization, LCM, DNA extraction, PCR reaction and sequencing were done according to Klitgaard et al. (2). The unknown invasive Spirochaetes species was recognized in pigs by using fluorescent in situ hybridization, followed by laser capture microdissection (LCM) of the targeted cells from the colonic mucosa. Direct 16S rRNA gene PCR was performed from the dissected micro-colonies. A phylogenetic analysis clustered the 16S rRNA gene in the Treponema genus group. The closest related Treponema type strain was T. bryantii (

AB - Helical-shaped bacteria resembling Spirochaetes commonly are present in the gastrointestinal tract of animals and humans. Culturing of Spirochaetes is in general fastidious and not always successful. Here, a new DNA isolation approach for prokaryotic cells in formalin-fixed paraffin-embedded tissue samples is applied for the identification of an unknown Spirochaete. The tissue samples from colon were obtained from a previous study (1). Sectioning, in situ hybridization, LCM, DNA extraction, PCR reaction and sequencing were done according to Klitgaard et al. (2). The unknown invasive Spirochaetes species was recognized in pigs by using fluorescent in situ hybridization, followed by laser capture microdissection (LCM) of the targeted cells from the colonic mucosa. Direct 16S rRNA gene PCR was performed from the dissected micro-colonies. A phylogenetic analysis clustered the 16S rRNA gene in the Treponema genus group. The closest related Treponema type strain was T. bryantii (

UR - https://orbit.dtu.dk/en/publications/6e60232d-8f69-48f0-8aea-0c72ffb6dd24

M3 - Conference abstract for conference

ER -

ID: 339890849