Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa
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Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa. / Yang, Liang; Liu, Yang; Markussen, Trine; Høiby, Niels; Tolker-Nielsen, Tim; Molin, Søren.
In: F E M S Immunology and Medical Microbiology, Vol. 62, No. 3, 01.08.2011, p. 339-47.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa
AU - Yang, Liang
AU - Liu, Yang
AU - Markussen, Trine
AU - Høiby, Niels
AU - Tolker-Nielsen, Tim
AU - Molin, Søren
N1 - © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
PY - 2011/8/1
Y1 - 2011/8/1
N2 - Biofilm infections may not simply be the result of colonization by one bacterium, but rather the consequence of pathogenic contributions from several bacteria. Interspecies interactions of different organisms in mixed-species biofilms remain largely unexplained, but knowledge of these is very important for understanding of biofilm physiology and the treatment of biofilm-related infectious diseases. Here, we have investigated interactions of two of the major bacterial species of cystic fibrosis lung microbial communities -Pseudomonas aeruginosa and Staphylococcus aureus- when grown in co-culture biofilms. By growing co-culture biofilms of S. aureus with P. aeruginosa mutants in a flow-chamber system and observing them using confocal laser scanning microscopy, we show that wild-type P. aeruginosa PAO1 facilitates S. aureus microcolony formation. In contrast, P. aeruginosa mucA and rpoN mutants do not facilitate S. aureus microcolony formation and tend to outcompete S. aureus in co-culture biofilms. Further investigations reveal that extracellular DNA (eDNA) plays an important role in S. aureus microcolony formation and that P. aeruginosa type IV pili are required for this process, probably through their ability to bind to eDNA. Furthermore, P. aeruginosa is able to protect S. aureus against Dictyostelium discoideum phagocytosis in co-culture biofilms.
AB - Biofilm infections may not simply be the result of colonization by one bacterium, but rather the consequence of pathogenic contributions from several bacteria. Interspecies interactions of different organisms in mixed-species biofilms remain largely unexplained, but knowledge of these is very important for understanding of biofilm physiology and the treatment of biofilm-related infectious diseases. Here, we have investigated interactions of two of the major bacterial species of cystic fibrosis lung microbial communities -Pseudomonas aeruginosa and Staphylococcus aureus- when grown in co-culture biofilms. By growing co-culture biofilms of S. aureus with P. aeruginosa mutants in a flow-chamber system and observing them using confocal laser scanning microscopy, we show that wild-type P. aeruginosa PAO1 facilitates S. aureus microcolony formation. In contrast, P. aeruginosa mucA and rpoN mutants do not facilitate S. aureus microcolony formation and tend to outcompete S. aureus in co-culture biofilms. Further investigations reveal that extracellular DNA (eDNA) plays an important role in S. aureus microcolony formation and that P. aeruginosa type IV pili are required for this process, probably through their ability to bind to eDNA. Furthermore, P. aeruginosa is able to protect S. aureus against Dictyostelium discoideum phagocytosis in co-culture biofilms.
KW - Biofilms
KW - Coculture Techniques
KW - DNA, Bacterial
KW - Dictyostelium
KW - Fimbriae Proteins
KW - Microscopy, Confocal
KW - Mutation
KW - Phagocytosis
KW - Pseudomonas aeruginosa
KW - RNA Polymerase Sigma 54
KW - Staphylococcus aureus
KW - Symbiosis
UR - http://dx.doi.org/10.1111/j.1574-695X.2011.00820.x
U2 - 10.1111/j.1574-695X.2011.00820.x
DO - 10.1111/j.1574-695X.2011.00820.x
M3 - Journal article
C2 - 21595754
VL - 62
SP - 339
EP - 347
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 3
ER -
ID: 36061655