Pathogenic CD8+ epidermis-resident memory T cells displace dendritic epidermal T cells in allergic dermatitis
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Pathogenic CD8+ epidermis-resident memory T cells displace dendritic epidermal T cells in allergic dermatitis. / Ø Gadsbøll, Anne-Sofie; Jee, Mia H; Funch, Anders B; Alhede, Maria; Mraz, Veronika; Weber, Julie F; Callender, Lauren A; Carroll, Elizabeth C; Bjarnsholt, Thomas; Woetmann, Anders; Ødum, Niels; Thomsen, Allan R; Johansen, Jeanne D; Henson, Sian M; Geisler, Carsten; Bonefeld, Charlotte M.
In: The Journal of Investigative Dermatology, Vol. 140, No. 4, 2020, p. 806-815.e5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pathogenic CD8+ epidermis-resident memory T cells displace dendritic epidermal T cells in allergic dermatitis
AU - Ø Gadsbøll, Anne-Sofie
AU - Jee, Mia H
AU - Funch, Anders B
AU - Alhede, Maria
AU - Mraz, Veronika
AU - Weber, Julie F
AU - Callender, Lauren A
AU - Carroll, Elizabeth C
AU - Bjarnsholt, Thomas
AU - Woetmann, Anders
AU - Ødum, Niels
AU - Thomsen, Allan R
AU - Johansen, Jeanne D
AU - Henson, Sian M
AU - Geisler, Carsten
AU - Bonefeld, Charlotte M
N1 - Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2020
Y1 - 2020
N2 - The skin is our interface with the outside world and consequently it is exposed to a wide range of microbes and allergens. Recent studies have indicated that allergen-specific skin-resident memory T (TRM) cells play a role in allergic contact dermatitis (ACD). However, the composition and dynamics of the epidermal T cell subsets during ACD are not known. Here we show that exposure of the skin to the experimental contact allergen 1-fluoro-2,4-dinitrobenzene (DNFB) results in a displacement of the normally occurring dendritic epidermal T cells (DETC) concomitant with an accumulation of epidermal CD8+CD69+CD103+ TRM cells in mice. By studying knock-out mice, we provide evidence that CD8+ T cells are required for the displacement of the DETC and furthermore, that DETC are not required for recruitment of CD8+ TRM cells to the epidermis following allergen exposure. We demonstrate that the magnitude of the allergic reaction correlates with the number of CD8+ epidermal TRM cells that again correlates with allergen dose and number of allergen exposures. Finally, in an attempt to elucidate why CD8+ epidermal TRM cells persist in the epidermis, we show that CD8+ epidermal TRM cells have a higher proliferative capability and are bioenergetically more stable, displaying a higher spare respiratory capacity than DETC.
AB - The skin is our interface with the outside world and consequently it is exposed to a wide range of microbes and allergens. Recent studies have indicated that allergen-specific skin-resident memory T (TRM) cells play a role in allergic contact dermatitis (ACD). However, the composition and dynamics of the epidermal T cell subsets during ACD are not known. Here we show that exposure of the skin to the experimental contact allergen 1-fluoro-2,4-dinitrobenzene (DNFB) results in a displacement of the normally occurring dendritic epidermal T cells (DETC) concomitant with an accumulation of epidermal CD8+CD69+CD103+ TRM cells in mice. By studying knock-out mice, we provide evidence that CD8+ T cells are required for the displacement of the DETC and furthermore, that DETC are not required for recruitment of CD8+ TRM cells to the epidermis following allergen exposure. We demonstrate that the magnitude of the allergic reaction correlates with the number of CD8+ epidermal TRM cells that again correlates with allergen dose and number of allergen exposures. Finally, in an attempt to elucidate why CD8+ epidermal TRM cells persist in the epidermis, we show that CD8+ epidermal TRM cells have a higher proliferative capability and are bioenergetically more stable, displaying a higher spare respiratory capacity than DETC.
U2 - 10.1016/j.jid.2019.07.722
DO - 10.1016/j.jid.2019.07.722
M3 - Journal article
C2 - 31518559
VL - 140
SP - 806-815.e5
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 4
ER -
ID: 227415252