Onset of transcription of the aminopeptidase N (leukemia antigen CD 13) gene at the crypt/villus transition zone during rabbit enterocyte differentiation.
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Onset of transcription of the aminopeptidase N (leukemia antigen CD 13) gene at the crypt/villus transition zone during rabbit enterocyte differentiation. / Norén, O; Dabelsteen, E; Høyer, P E; Sjöström, H; Olsen, Jørgen; Hansen, Gert Helge.
In: FEBS Letters, Vol. 259, No. 1, 1989, p. 107-12.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Onset of transcription of the aminopeptidase N (leukemia antigen CD 13) gene at the crypt/villus transition zone during rabbit enterocyte differentiation.
AU - Norén, O
AU - Dabelsteen, E
AU - Høyer, P E
AU - Sjöström, H
AU - Olsen, Jørgen
AU - Hansen, Gert Helge
N1 - Keywords: Amino Acid Sequence; Aminopeptidases; Animals; Antigens, CD13; Antigens, Differentiation, Myelomonocytic; Base Sequence; Blotting, Northern; Cell Differentiation; Gene Expression Regulation, Enzymologic; Intestine, Small; Microvilli; Molecular Sequence Data; Nucleic Acid Hybridization; RNA Probes; Rabbits; Transcription, Genetic
PY - 1989
Y1 - 1989
N2 - The sequence of a cDNA clone (2.82 kbp) of rabbit intestinal aminopeptidase N (CD 13) is reported. Using the corresponding anti-sense RNA probe, the distribution of aminopeptidase N mRNA along the crypt/villus axis of the rabbit small intestine was studied by in situ hybridization. The aminopeptidase N gene is expressed along the whole length of the villus with a maximum at its base. Expression was not detected in the crypt cells. The distribution of aminopeptidase N mRNA correlates with the presence of active enzyme as monitored by histochemical staining. The results are compatible with onset of transcription of the aminopeptidase N gene at the crypt/villus transition zone during the enterocyte differentiation.
AB - The sequence of a cDNA clone (2.82 kbp) of rabbit intestinal aminopeptidase N (CD 13) is reported. Using the corresponding anti-sense RNA probe, the distribution of aminopeptidase N mRNA along the crypt/villus axis of the rabbit small intestine was studied by in situ hybridization. The aminopeptidase N gene is expressed along the whole length of the villus with a maximum at its base. Expression was not detected in the crypt cells. The distribution of aminopeptidase N mRNA correlates with the presence of active enzyme as monitored by histochemical staining. The results are compatible with onset of transcription of the aminopeptidase N gene at the crypt/villus transition zone during the enterocyte differentiation.
M3 - Journal article
C2 - 2574692
VL - 259
SP - 107
EP - 112
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1
ER -
ID: 6586536