Non-invasive method for sampling and extraction of mouse DNA for PCR

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Standard

Non-invasive method for sampling and extraction of mouse DNA for PCR. / Meldgaard, M; Bollen, P J A; Finsen, B.

In: Laboratory Animals, Vol. 38, No. 4, 2004, p. 413-417.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Meldgaard, M, Bollen, PJA & Finsen, B 2004, 'Non-invasive method for sampling and extraction of mouse DNA for PCR', Laboratory Animals, vol. 38, no. 4, pp. 413-417. https://doi.org/10.1258/0023677041958981

APA

Meldgaard, M., Bollen, P. J. A., & Finsen, B. (2004). Non-invasive method for sampling and extraction of mouse DNA for PCR. Laboratory Animals, 38(4), 413-417. https://doi.org/10.1258/0023677041958981

Vancouver

Meldgaard M, Bollen PJA, Finsen B. Non-invasive method for sampling and extraction of mouse DNA for PCR. Laboratory Animals. 2004;38(4):413-417. https://doi.org/10.1258/0023677041958981

Author

Meldgaard, M ; Bollen, P J A ; Finsen, B. / Non-invasive method for sampling and extraction of mouse DNA for PCR. In: Laboratory Animals. 2004 ; Vol. 38, No. 4. pp. 413-417.

Bibtex

@article{7753c0281d684668a85b5a8640525c0f,
title = "Non-invasive method for sampling and extraction of mouse DNA for PCR",
abstract = "We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.",
keywords = "Animals, Cheek/physiology, DNA/chemistry, Epithelial Cells/chemistry, Mice/genetics, Polymerase Chain Reaction/methods, Saliva/chemistry, Specimen Handling/methods",
author = "M Meldgaard and Bollen, {P J A} and B Finsen",
year = "2004",
doi = "10.1258/0023677041958981",
language = "English",
volume = "38",
pages = "413--417",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications",
number = "4",

}

RIS

TY - JOUR

T1 - Non-invasive method for sampling and extraction of mouse DNA for PCR

AU - Meldgaard, M

AU - Bollen, P J A

AU - Finsen, B

PY - 2004

Y1 - 2004

N2 - We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.

AB - We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.

KW - Animals

KW - Cheek/physiology

KW - DNA/chemistry

KW - Epithelial Cells/chemistry

KW - Mice/genetics

KW - Polymerase Chain Reaction/methods

KW - Saliva/chemistry

KW - Specimen Handling/methods

U2 - 10.1258/0023677041958981

DO - 10.1258/0023677041958981

M3 - Journal article

C2 - 15479556

VL - 38

SP - 413

EP - 417

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 4

ER -

ID: 324128689