New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration
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New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration. / Martin, L.; Ashenden, M; Bejder, Jacob; Hoffmann, M; Nordsborg, Nikolai Baastrup; Karstoft, K; Mørkeberg, Jakob Sehested; Sharpe, K; Lasne, F; Marchand, A.
In: Drug Testing and Analysis, Vol. 8, No. 11-12, 2016, p. 1119-1130.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration
AU - Martin, L.
AU - Ashenden, M
AU - Bejder, Jacob
AU - Hoffmann, M
AU - Nordsborg, Nikolai Baastrup
AU - Karstoft, K
AU - Mørkeberg, Jakob Sehested
AU - Sharpe, K
AU - Lasne, F
AU - Marchand, A
N1 - CURIS 2016 NEXS 407
PY - 2016
Y1 - 2016
N2 - To minimize the chances of being caught after doping with recombinant human erythropoietins (rhEPO), athletes have turned to new practices using micro-doses and excess fluid ingestion to accelerate elimination and decrease the probability of detection. Our objective was to test the sensitivity of detection by validated methods (IEF: isoelectric focusing; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis) when such practices are used. First, after a three-week rhEPO boost period and 10 days of wash out, detection of a single 900 IU micro-dose of Eprex® was evaluated in healthy male subjects. After an injection in the evening, urine and plasma samples were collected the following morning. Half of the subjects then drank a bolus of water and new samples were collected 80 min later. Interestingly, rhEPO was detected in 100% of the samples even after water ingestion. A second similar protocol was then performed with a single injection of a micro-dose of rhEPO (500 IU or 900 IU), without a prior rhEPO boost. In addition, urine and plasma samples were also collected 15 and 20 h post rhEPO administration. Once again drinking water did not affect the rate of detection. Urine appeared a better matrix to detect micro-doses after 10 h, enabling between 92% and 100% of identification at that time. The rate of identification decreased rapidly thereafter, in particular for the 500 IU micro-dose. However IEF analysis still resulted in 71% identification of rhEPO in urine after 20 h. These results could help to define a better strategy for controlling and identifying athletes using rhEPO micro-doses.
AB - To minimize the chances of being caught after doping with recombinant human erythropoietins (rhEPO), athletes have turned to new practices using micro-doses and excess fluid ingestion to accelerate elimination and decrease the probability of detection. Our objective was to test the sensitivity of detection by validated methods (IEF: isoelectric focusing; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis) when such practices are used. First, after a three-week rhEPO boost period and 10 days of wash out, detection of a single 900 IU micro-dose of Eprex® was evaluated in healthy male subjects. After an injection in the evening, urine and plasma samples were collected the following morning. Half of the subjects then drank a bolus of water and new samples were collected 80 min later. Interestingly, rhEPO was detected in 100% of the samples even after water ingestion. A second similar protocol was then performed with a single injection of a micro-dose of rhEPO (500 IU or 900 IU), without a prior rhEPO boost. In addition, urine and plasma samples were also collected 15 and 20 h post rhEPO administration. Once again drinking water did not affect the rate of detection. Urine appeared a better matrix to detect micro-doses after 10 h, enabling between 92% and 100% of identification at that time. The rate of identification decreased rapidly thereafter, in particular for the 500 IU micro-dose. However IEF analysis still resulted in 71% identification of rhEPO in urine after 20 h. These results could help to define a better strategy for controlling and identifying athletes using rhEPO micro-doses.
KW - Doping
KW - EPO
KW - IEF
KW - Micro-doses
KW - SDS-PAGE
U2 - 10.1002/dta.2004
DO - 10.1002/dta.2004
M3 - Journal article
C2 - 27390252
AN - SCOPUS:84978245072
VL - 8
SP - 1119
EP - 1130
JO - Drug Testing and Analysis
JF - Drug Testing and Analysis
SN - 1942-7603
IS - 11-12
ER -
ID: 179174510