Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique. / Genina, Natalja; Räikkönen, Heikki; Heinämäki, Jyrki; Veski, Peep; Yliruusi, Jouko.

In: AAPS PharmSciTech, Vol. 11, No. 2, 2010, p. 959-965.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Genina, N, Räikkönen, H, Heinämäki, J, Veski, P & Yliruusi, J 2010, 'Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique.', AAPS PharmSciTech, vol. 11, no. 2, pp. 959-965. https://doi.org/10.1208/s12249-010-9462-4

APA

Genina, N., Räikkönen, H., Heinämäki, J., Veski, P., & Yliruusi, J. (2010). Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique. AAPS PharmSciTech, 11(2), 959-965. https://doi.org/10.1208/s12249-010-9462-4

Vancouver

Genina N, Räikkönen H, Heinämäki J, Veski P, Yliruusi J. Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique. AAPS PharmSciTech. 2010;11(2):959-965. https://doi.org/10.1208/s12249-010-9462-4

Author

Genina, Natalja ; Räikkönen, Heikki ; Heinämäki, Jyrki ; Veski, Peep ; Yliruusi, Jouko. / Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique. In: AAPS PharmSciTech. 2010 ; Vol. 11, No. 2. pp. 959-965.

Bibtex

@article{4b02299a7c874cf8a7f418dcdd7be6e6,
title = "Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique.",
abstract = "We nano-coated powdered lactose particles with the enzyme beta-galactosidase using an ultrasound-assisted technique. Atomization of the enzyme solution did not change its activity. The amount of surface-attached beta-galactosidase was measured through its enzymatic reaction product D-galactose using a standardized method. A near-linear increase was obtained in the thickness of the enzyme coat as the treatment proceeded. Interestingly, lactose, which is a substrate for beta-galactosidase, did not undergo enzymatic degradation during processing and remained unchanged for at least 1 month. Stability of protein-coated lactose was due to the absence of water within the powder, as it was dry after the treatment procedure. In conclusion, we were able to attach the polypeptide to the core particles and determine precisely the coating efficiency of the surface-treated powder using a simple approach.",
keywords = "lactose, nano-coating, protein, ultrasound, β -galactosidase",
author = "Natalja Genina and Heikki R{\"a}ikk{\"o}nen and Jyrki Hein{\"a}m{\"a}ki and Peep Veski and Jouko Yliruusi",
year = "2010",
doi = "10.1208/s12249-010-9462-4",
language = "English",
volume = "11",
pages = "959--965",
journal = "AAPS PharmSciTech",
issn = "1530-9932",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique.

AU - Genina, Natalja

AU - Räikkönen, Heikki

AU - Heinämäki, Jyrki

AU - Veski, Peep

AU - Yliruusi, Jouko

PY - 2010

Y1 - 2010

N2 - We nano-coated powdered lactose particles with the enzyme beta-galactosidase using an ultrasound-assisted technique. Atomization of the enzyme solution did not change its activity. The amount of surface-attached beta-galactosidase was measured through its enzymatic reaction product D-galactose using a standardized method. A near-linear increase was obtained in the thickness of the enzyme coat as the treatment proceeded. Interestingly, lactose, which is a substrate for beta-galactosidase, did not undergo enzymatic degradation during processing and remained unchanged for at least 1 month. Stability of protein-coated lactose was due to the absence of water within the powder, as it was dry after the treatment procedure. In conclusion, we were able to attach the polypeptide to the core particles and determine precisely the coating efficiency of the surface-treated powder using a simple approach.

AB - We nano-coated powdered lactose particles with the enzyme beta-galactosidase using an ultrasound-assisted technique. Atomization of the enzyme solution did not change its activity. The amount of surface-attached beta-galactosidase was measured through its enzymatic reaction product D-galactose using a standardized method. A near-linear increase was obtained in the thickness of the enzyme coat as the treatment proceeded. Interestingly, lactose, which is a substrate for beta-galactosidase, did not undergo enzymatic degradation during processing and remained unchanged for at least 1 month. Stability of protein-coated lactose was due to the absence of water within the powder, as it was dry after the treatment procedure. In conclusion, we were able to attach the polypeptide to the core particles and determine precisely the coating efficiency of the surface-treated powder using a simple approach.

KW - lactose

KW - nano-coating

KW - protein

KW - ultrasound

KW - β -galactosidase

U2 - 10.1208/s12249-010-9462-4

DO - 10.1208/s12249-010-9462-4

M3 - Journal article

C2 - 20512434

VL - 11

SP - 959

EP - 965

JO - AAPS PharmSciTech

JF - AAPS PharmSciTech

SN - 1530-9932

IS - 2

ER -

ID: 145538492