Midline 1 controls polarization and migration of murine cytotoxic T cells
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Midline 1 controls polarization and migration of murine cytotoxic T cells. / Boding, Lasse; Hansen, Ann K; Nielsen, Morten M; Meroni, Germana; Braunstein, Thomas H; Woetmann, Anders; Ødum, Niels; Bonefeld, Charlotte M; Geisler, Carsten.
In: Immunity, Inflammation and Disease, Vol. 2, No. 4, 12.2014, p. 262-71.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Midline 1 controls polarization and migration of murine cytotoxic T cells
AU - Boding, Lasse
AU - Hansen, Ann K
AU - Nielsen, Morten M
AU - Meroni, Germana
AU - Braunstein, Thomas H
AU - Woetmann, Anders
AU - Ødum, Niels
AU - Bonefeld, Charlotte M
AU - Geisler, Carsten
PY - 2014/12
Y1 - 2014/12
N2 - Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2 A levels. Loss-of-function mutations in MID1 lead to the human X-linked Opitz G/BBB (OS) syndrome characterized by defective midline development during embryogenesis. We have recently shown that MID1 is strongly up-regulated in murine cytotoxic T lymphocytes (CTLs), and that it has a significant impact on exocytosis of lytic granules and the killing capacity of CTLs. The aims of the present study were to determine the localization of MID1 in migrating CTLs, and to investigate whether MID1 affects CTL polarization and migration. We found that MID1 mainly localizes to the uropod of migrating CTLs and that it has a substantial impact on CTL polarization and migration in vitro. Furthermore, analysis of contact hypersensitivity responses supported that MID1 controls effector functions of CTLs in hapten-challenged skin in vivo. These results provide significant new knowledge on the role of MID1 in CTL biology.
AB - Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2 A levels. Loss-of-function mutations in MID1 lead to the human X-linked Opitz G/BBB (OS) syndrome characterized by defective midline development during embryogenesis. We have recently shown that MID1 is strongly up-regulated in murine cytotoxic T lymphocytes (CTLs), and that it has a significant impact on exocytosis of lytic granules and the killing capacity of CTLs. The aims of the present study were to determine the localization of MID1 in migrating CTLs, and to investigate whether MID1 affects CTL polarization and migration. We found that MID1 mainly localizes to the uropod of migrating CTLs and that it has a substantial impact on CTL polarization and migration in vitro. Furthermore, analysis of contact hypersensitivity responses supported that MID1 controls effector functions of CTLs in hapten-challenged skin in vivo. These results provide significant new knowledge on the role of MID1 in CTL biology.
U2 - 10.1002/iid3.44
DO - 10.1002/iid3.44
M3 - Journal article
C2 - 25866633
VL - 2
SP - 262
EP - 271
JO - Immunity, inflammation and disease
JF - Immunity, inflammation and disease
SN - 2050-4527
IS - 4
ER -
ID: 135490914