Localization in the gastrointestinal tract of immunoreactive prosomatostatin
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Localization in the gastrointestinal tract of immunoreactive prosomatostatin. / Holst, J J; Poulsen, Steen Seier.
In: Regulatory Peptides, Vol. 19, No. 3-4, 11.1987, p. 183-95.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Localization in the gastrointestinal tract of immunoreactive prosomatostatin
AU - Holst, J J
AU - Poulsen, Steen Seier
PY - 1987/11
Y1 - 1987/11
N2 - Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.
AB - Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.
KW - Animals
KW - Chromatography, Gel
KW - Colon
KW - Digestive System
KW - Duodenum
KW - Ileum
KW - Immunohistochemistry
KW - Jejunum
KW - Protein Precursors
KW - Radioimmunoassay
KW - Somatostatin
KW - Stomach
KW - Swine
M3 - Journal article
C2 - 2893436
VL - 19
SP - 183
EP - 195
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 3-4
ER -
ID: 47488519