TY - JOUR

T1 - Limited anti-HIV neutralizing antibody breadth and potency before and after HIV superinfection in Danish men who have sex with men

AU - Redd, Andrew D

AU - Helleberg, Marie

AU - Sievers, Matthew

AU - Schmidt, Stephen D

AU - Doria-Rose, Nicole A

AU - Bruno, Daniel

AU - Traeger, Shelby

AU - Martens, Craig

AU - Fonager, Jannik

AU - Kronborg, Gitte

AU - Packman, Zoe

AU - Mascola, John R

AU - Porcella, Stephen F

AU - Gerstoft, Jan

AU - Quinn, Thomas C

PY - 2019/1

Y1 - 2019/1

N2 - BACKGROUND: The role of the anti-HIV neutralizing antibody response in protecting against HIV superinfection, and changes in neutralizing antibody potency and breadth after HIV superinfection have not been fully elucidated. This study examined the rate of HIV superinfection in men who have sex with men (MSM) also diagnosed with syphilis in Denmark, and the anti-HIV neutralizing antibody response in men who became superinfected.MATERIALS AND METHODS: MSM enrolled in the Danish HIV cohort who acquired syphilis were examined longitudinally for HIV superinfection using a validated next-generation sequencing assay. HIV superinfection cases were matched 3:1 to controls, and neutralizing antibody responses before (cases/controls) and after (cases) HIV superinfection were determined using a 20-pseudovirus panel.RESULTS: Four cases of HIV superinfection were identified from 95 MSM screened for a rate of HIV superinfection of 1.56/100 pys (95% CI = 0.43-4.01). Prior to HIV superinfection neutralizing antibody responses were low in breadth and potency, and did not differ between cases and controls (p = 1.0). In cases, neutralizing antibody responses increased modestly after HIV superinfection.CONCLUSIONS: These data support the theory that the natural neutralizing antibody response to HIV infection may not be the controlling factor in protecting against a subsequent HIV challenge.

AB - BACKGROUND: The role of the anti-HIV neutralizing antibody response in protecting against HIV superinfection, and changes in neutralizing antibody potency and breadth after HIV superinfection have not been fully elucidated. This study examined the rate of HIV superinfection in men who have sex with men (MSM) also diagnosed with syphilis in Denmark, and the anti-HIV neutralizing antibody response in men who became superinfected.MATERIALS AND METHODS: MSM enrolled in the Danish HIV cohort who acquired syphilis were examined longitudinally for HIV superinfection using a validated next-generation sequencing assay. HIV superinfection cases were matched 3:1 to controls, and neutralizing antibody responses before (cases/controls) and after (cases) HIV superinfection were determined using a 20-pseudovirus panel.RESULTS: Four cases of HIV superinfection were identified from 95 MSM screened for a rate of HIV superinfection of 1.56/100 pys (95% CI = 0.43-4.01). Prior to HIV superinfection neutralizing antibody responses were low in breadth and potency, and did not differ between cases and controls (p = 1.0). In cases, neutralizing antibody responses increased modestly after HIV superinfection.CONCLUSIONS: These data support the theory that the natural neutralizing antibody response to HIV infection may not be the controlling factor in protecting against a subsequent HIV challenge.

KW - Antibodies, Neutralizing/blood

KW - Denmark

KW - HIV/classification

KW - HIV Antibodies/blood

KW - HIV Infections/immunology

KW - High-Throughput Nucleotide Sequencing

KW - Homosexuality, Male

KW - Humans

KW - Longitudinal Studies

KW - Male

KW - Prospective Studies

KW - Superinfection/immunology

KW - Syphilis/complications

U2 - 10.1080/23744235.2018.1500708

DO - 10.1080/23744235.2018.1500708

M3 - Journal article

C2 - 30317905

VL - 51

SP - 56

EP - 61

JO - Infectious Diseases

JF - Infectious Diseases

SN - 2374-4235

IS - 1

ER -