Lessons learned from histological step-sectioning of sentinel lymph nodes in penile cancer
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Lessons learned from histological step-sectioning of sentinel lymph nodes in penile cancer. / Elversang, Johanna; Grønkær Toft, Birgitte; Predbjørn Krarup, Kim; Jakobsen, Jakob K.
In: Histopathology, Vol. 78, No. 4, 2021, p. 627-633.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lessons learned from histological step-sectioning of sentinel lymph nodes in penile cancer
AU - Elversang, Johanna
AU - Grønkær Toft, Birgitte
AU - Predbjørn Krarup, Kim
AU - Jakobsen, Jakob K.
N1 - Publisher Copyright: © 2020 John Wiley & Sons Ltd
PY - 2021
Y1 - 2021
N2 - Aims: Histopathological examination of sentinel lymph nodes in penile cancer has varied during the first 10 years after introduction of the dynamic sentinel node (SLN) procedure in Denmark, and guidelines have been sparse. The aim of this study was to investigate the impact of rigorous step-sectioning of sentinel lymph nodes in penile cancer and improve guidelines. Methods and results: Seventy-two penile squamous cell carcinoma patients undergoing SLN procedure at a single institution in 2000–2010 were included. The archived lymph node tissues already subjected to a standard pathological examination were retrieved and the initially negative lymph nodes were subjected to an extended step-sectioning procedure. The results were compared to clinical patient outcome from a national database and subsequent pathology reports. The original histopathological examination had detected 26 SLN with metastasis, 21 macro metastases and five micro metastases. The additional step-sectioning procedure of this study generated 4606 slides; seven SLN metastases, two macro metastases and five micro metastases, were detected. One of the macro metastases originated from a patient in which a clinical relapse had occurred in the same groin. None of the other metastases detected in this study showed ipsilateral relapse during follow-up. Conclusion: The results underline the value of our current practice of step-sectioning sentinel lymph nodes in penile cancer and the need for histopathological routines and guidelines. The Danish national guidelines on histopathological handling of sentinel lymph nodes have been adapted to detect any potential clinically relevant metastases.
AB - Aims: Histopathological examination of sentinel lymph nodes in penile cancer has varied during the first 10 years after introduction of the dynamic sentinel node (SLN) procedure in Denmark, and guidelines have been sparse. The aim of this study was to investigate the impact of rigorous step-sectioning of sentinel lymph nodes in penile cancer and improve guidelines. Methods and results: Seventy-two penile squamous cell carcinoma patients undergoing SLN procedure at a single institution in 2000–2010 were included. The archived lymph node tissues already subjected to a standard pathological examination were retrieved and the initially negative lymph nodes were subjected to an extended step-sectioning procedure. The results were compared to clinical patient outcome from a national database and subsequent pathology reports. The original histopathological examination had detected 26 SLN with metastasis, 21 macro metastases and five micro metastases. The additional step-sectioning procedure of this study generated 4606 slides; seven SLN metastases, two macro metastases and five micro metastases, were detected. One of the macro metastases originated from a patient in which a clinical relapse had occurred in the same groin. None of the other metastases detected in this study showed ipsilateral relapse during follow-up. Conclusion: The results underline the value of our current practice of step-sectioning sentinel lymph nodes in penile cancer and the need for histopathological routines and guidelines. The Danish national guidelines on histopathological handling of sentinel lymph nodes have been adapted to detect any potential clinically relevant metastases.
KW - lymph node metastases
KW - penile squamous cell carcinoma (PSCC)
KW - sentinel node biopsy
KW - step-sectioning
UR - http://www.scopus.com/inward/record.url?scp=85096923634&partnerID=8YFLogxK
U2 - 10.1111/his.14261
DO - 10.1111/his.14261
M3 - Journal article
C2 - 32979281
AN - SCOPUS:85096923634
VL - 78
SP - 627
EP - 633
JO - Histopathology
JF - Histopathology
SN - 0309-0167
IS - 4
ER -
ID: 270552780