Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. / Collins, Russell T.; Coxam, Baptiste; Fechner, Ines; Unterweger, Iris; Szymborska, Anna; Meier, Katja; Gerhardt, Holger.

In: Genesis, Vol. 58, No. 10-11, 23391, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Collins, RT, Coxam, B, Fechner, I, Unterweger, I, Szymborska, A, Meier, K & Gerhardt, H 2020, 'Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines', Genesis, vol. 58, no. 10-11, 23391. https://doi.org/10.1002/dvg.23391

APA

Collins, R. T., Coxam, B., Fechner, I., Unterweger, I., Szymborska, A., Meier, K., & Gerhardt, H. (2020). Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. Genesis, 58(10-11), [23391]. https://doi.org/10.1002/dvg.23391

Vancouver

Collins RT, Coxam B, Fechner I, Unterweger I, Szymborska A, Meier K et al. Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. Genesis. 2020;58(10-11). 23391. https://doi.org/10.1002/dvg.23391

Author

Collins, Russell T. ; Coxam, Baptiste ; Fechner, Ines ; Unterweger, Iris ; Szymborska, Anna ; Meier, Katja ; Gerhardt, Holger. / Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. In: Genesis. 2020 ; Vol. 58, No. 10-11.

Bibtex

@article{f9a1a3458f4b43b2a49768e7e3edef12,
title = "Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines",
abstract = "The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within theknock-insequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafishfli1agene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart.",
keywords = "animal model, CRISPR, Cas9, genome editing, screening, transgenesis, zebrafish",
author = "Collins, {Russell T.} and Baptiste Coxam and Ines Fechner and Iris Unterweger and Anna Szymborska and Katja Meier and Holger Gerhardt",
year = "2020",
doi = "10.1002/dvg.23391",
language = "English",
volume = "58",
journal = "Genesis: The Journal of Genetics and Development (Print)",
issn = "1526-954X",
publisher = "JohnWiley & Sons, Inc.",
number = "10-11",

}

RIS

TY - JOUR

T1 - Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines

AU - Collins, Russell T.

AU - Coxam, Baptiste

AU - Fechner, Ines

AU - Unterweger, Iris

AU - Szymborska, Anna

AU - Meier, Katja

AU - Gerhardt, Holger

PY - 2020

Y1 - 2020

N2 - The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within theknock-insequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafishfli1agene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart.

AB - The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within theknock-insequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafishfli1agene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart.

KW - animal model

KW - CRISPR

KW - Cas9

KW - genome editing

KW - screening

KW - transgenesis

KW - zebrafish

U2 - 10.1002/dvg.23391

DO - 10.1002/dvg.23391

M3 - Journal article

C2 - 32783355

VL - 58

JO - Genesis: The Journal of Genetics and Development (Print)

JF - Genesis: The Journal of Genetics and Development (Print)

SN - 1526-954X

IS - 10-11

M1 - 23391

ER -

ID: 280117922