Insulin-like growth factor I and glucagon-like peptide-2 responses to fasting followed by controlled or ad libitum refeeding in rats
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Insulin-like growth factor I and glucagon-like peptide-2 responses to fasting followed by controlled or ad libitum refeeding in rats. / Nelson, David W; Murali, Sangita G; Liu, Xiaowen; Koopmann, Matthew C; Holst, Jens Juul; Ney, Denise M.
In: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Vol. 294, No. 4, 04.2008, p. R1175-84.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Insulin-like growth factor I and glucagon-like peptide-2 responses to fasting followed by controlled or ad libitum refeeding in rats
AU - Nelson, David W
AU - Murali, Sangita G
AU - Liu, Xiaowen
AU - Koopmann, Matthew C
AU - Holst, Jens Juul
AU - Ney, Denise M
PY - 2008/4
Y1 - 2008/4
N2 - Luminal nutrients stimulate structural and functional regeneration in the intestine through mechanisms thought to involve insulin-like growth factor I (IGF-I) and glucagon-like peptide-2 (GLP-2). We investigated the relationship between IGF-I and GLP-2 responses and mucosal growth in rats fasted for 48 h and then refed for 2 or 4 days by continuous intravenous or intragastric infusion or ad libitum feeding. Fasting induced significant decreases in body weight, plasma concentrations of IGF-I and bioactive GLP-2, jejunal mucosal cellularity (mass, protein, DNA, and villus height), IGF-I mRNA, and ileal proglucagon mRNA. Plasma IGF-I concentration was restored to fed levels with 2 days of ad libitum refeeding but not with 4 days of intravenous or intragastric refeeding. Administration of an inhibitor of endogenous GLP-2 (rat GLP-2 3-33) during ad libitum refeeding partially attenuated mucosal growth and prevented the increase in plasma IGF-I to fed levels; however, plasma GLP-2 and jejunal IGF-I mRNA were restored to fed levels. Intragastric refeeding restored intestinal cellularity and functional capacity (sucrase activity and sodium-glucose transporter-1 expression) to fed levels, whereas intravenous refeeding had no effect. Intestinal regeneration after 4 days of intragastric or 2 days of ad libitum refeeding was positively associated with increases in plasma concentrations of GLP-2 and jejunal IGF-I mRNA. These data suggest that luminal nutrients stimulate intestinal growth, in part, by increased expression of both GLP-2 and IGF-I.
AB - Luminal nutrients stimulate structural and functional regeneration in the intestine through mechanisms thought to involve insulin-like growth factor I (IGF-I) and glucagon-like peptide-2 (GLP-2). We investigated the relationship between IGF-I and GLP-2 responses and mucosal growth in rats fasted for 48 h and then refed for 2 or 4 days by continuous intravenous or intragastric infusion or ad libitum feeding. Fasting induced significant decreases in body weight, plasma concentrations of IGF-I and bioactive GLP-2, jejunal mucosal cellularity (mass, protein, DNA, and villus height), IGF-I mRNA, and ileal proglucagon mRNA. Plasma IGF-I concentration was restored to fed levels with 2 days of ad libitum refeeding but not with 4 days of intravenous or intragastric refeeding. Administration of an inhibitor of endogenous GLP-2 (rat GLP-2 3-33) during ad libitum refeeding partially attenuated mucosal growth and prevented the increase in plasma IGF-I to fed levels; however, plasma GLP-2 and jejunal IGF-I mRNA were restored to fed levels. Intragastric refeeding restored intestinal cellularity and functional capacity (sucrase activity and sodium-glucose transporter-1 expression) to fed levels, whereas intravenous refeeding had no effect. Intestinal regeneration after 4 days of intragastric or 2 days of ad libitum refeeding was positively associated with increases in plasma concentrations of GLP-2 and jejunal IGF-I mRNA. These data suggest that luminal nutrients stimulate intestinal growth, in part, by increased expression of both GLP-2 and IGF-I.
KW - Adaptation, Physiological
KW - Animals
KW - Body Weight
KW - Dose-Response Relationship, Drug
KW - Eating
KW - Fasting
KW - Glucagon-Like Peptide 2
KW - Ileum
KW - Infusions, Intravenous
KW - Insulin
KW - Insulin-Like Growth Factor Binding Protein 3
KW - Insulin-Like Growth Factor Binding Protein 5
KW - Insulin-Like Growth Factor I
KW - Intestinal Mucosa
KW - Intubation, Gastrointestinal
KW - Jejunum
KW - Male
KW - Nitrogen
KW - Parenteral Nutrition
KW - Peptide Fragments
KW - Proglucagon
KW - RNA, Messenger
KW - Rats
KW - Rats, Sprague-Dawley
KW - Regeneration
KW - Sodium-Glucose Transporter 1
KW - Sucrase
KW - Time Factors
U2 - 10.1152/ajpregu.00238.2007
DO - 10.1152/ajpregu.00238.2007
M3 - Journal article
C2 - 18256135
VL - 294
SP - R1175-84
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 4
ER -
ID: 132049370