Induction of rat yolk sac carcinomas with consistent pattern of laminin, entactin, and type IV collagen biosynthesis
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Induction of rat yolk sac carcinomas with consistent pattern of laminin, entactin, and type IV collagen biosynthesis. / Wewer, U.
In: Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology, Vol. 92, No. 4, 01.07.1984, p. 275-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Induction of rat yolk sac carcinomas with consistent pattern of laminin, entactin, and type IV collagen biosynthesis
AU - Wewer, U
PY - 1984/7/1
Y1 - 1984/7/1
N2 - Twenty-four yolk sac carcinomas in Lewis rats were experimentally induced by puncturing the pregnant uterine wall with a hypodermic needle at day 9-13 of gestation. Morphologically, the tumours were composed of parietal- and visceral yolk sac carcinoma and to a less degree of trophoblastic giant cells. The tumours were adapted to ascitic growth and tissue culture, but eventually became committed to express only parietal endoderm phenotype. Immunohistochemical and metabolic labelling studies on the established lines in vivo and in vitro revealed a consistent pattern with respect to their biosynthesis of the basement membrane components laminin, entactin, and type IV collagen. This model system offers a simple approach to inducing rat yolk sac carcinomas for further morphological and biochemical characterization of the basement membrane.
AB - Twenty-four yolk sac carcinomas in Lewis rats were experimentally induced by puncturing the pregnant uterine wall with a hypodermic needle at day 9-13 of gestation. Morphologically, the tumours were composed of parietal- and visceral yolk sac carcinoma and to a less degree of trophoblastic giant cells. The tumours were adapted to ascitic growth and tissue culture, but eventually became committed to express only parietal endoderm phenotype. Immunohistochemical and metabolic labelling studies on the established lines in vivo and in vitro revealed a consistent pattern with respect to their biosynthesis of the basement membrane components laminin, entactin, and type IV collagen. This model system offers a simple approach to inducing rat yolk sac carcinomas for further morphological and biochemical characterization of the basement membrane.
KW - Animals
KW - Basement Membrane
KW - Cell Line
KW - Collagen
KW - Dysgerminoma
KW - Female
KW - Glycoproteins
KW - Histocytochemistry
KW - Immunochemistry
KW - Laminin
KW - Male
KW - Membrane Glycoproteins
KW - Neoplasm Proteins
KW - Neoplasm Transplantation
KW - Rats
KW - Rats, Inbred Lew
M3 - Journal article
C2 - 6333777
VL - 92
SP - 275
EP - 283
JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section A Pathology
JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section A Pathology
SN - 0108-0164
IS - 4
ER -
ID: 34330360