In vitro Assay to Evaluate Cation Transport of Ionophores
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In vitro Assay to Evaluate Cation Transport of Ionophores. / Uzun, Huriye; Vázquez-Hernández, Melissa; Bandow, Julia; Pomorski, Thomas.
In: Bio-protocol, Vol. 12, No. 22, e4552, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro Assay to Evaluate Cation Transport of Ionophores
AU - Uzun, Huriye
AU - Vázquez-Hernández, Melissa
AU - Bandow, Julia
AU - Pomorski, Thomas
PY - 2022
Y1 - 2022
N2 - Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.
AB - Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.
U2 - 10.21769/BioProtoc.4552
DO - 10.21769/BioProtoc.4552
M3 - Journal article
C2 - 36532682
VL - 12
JO - Bio-protocol
JF - Bio-protocol
SN - 2331-8325
IS - 22
M1 - e4552
ER -
ID: 337988383