In vitro ability of mastitis causing pathogens to form biofilms
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In vitro ability of mastitis causing pathogens to form biofilms. / Schönborn, Sarah; Wente, Nicole; Paduch, Jan-Hendrik; Krömker, Volker.
In: Journal of Dairy Research, Vol. 84, No. 2, 05.2017, p. 198-201.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro ability of mastitis causing pathogens to form biofilms
AU - Schönborn, Sarah
AU - Wente, Nicole
AU - Paduch, Jan-Hendrik
AU - Krömker, Volker
PY - 2017/5
Y1 - 2017/5
N2 - This Research Communication describes the study of in vitro biofilm formation of mastitis causing pathogens. Biofilms are communities of bacteria that are attached to a surface and to each other and are embedded in a self-produced matrix of extracellular polymeric substances. Biofilm formation is an important virulence factor that may result in recurrent or persistent udder infections and treatment failure through increased resistance to antibiotics and protection against host defences. In the present study 252 bacterial isolates from milk samples from bovine udder quarters with intramammary infections were examined with Congo Red agar (CRA) method and tube method (TM) for their ability to form biofilms. Both tests revealed a high number of biofilm-positive strains. Literature reports that the cure rates for Staphylococcus aureus infected udders are lower (27%) in comparison to cure rates of Streptococcus uberis (64-81%) or coagulase-negative staphylococci (CNS) mastitis (80-90%). The findings of the present study suggest that biofilm formation is not the main factor for the differences in cure rates of the various bacteria genera, because all tested pathogen groups showed a similarly high proportion of biofilm formation. Further research is needed to detect microbial biofilms on bovine udder epithelia.
AB - This Research Communication describes the study of in vitro biofilm formation of mastitis causing pathogens. Biofilms are communities of bacteria that are attached to a surface and to each other and are embedded in a self-produced matrix of extracellular polymeric substances. Biofilm formation is an important virulence factor that may result in recurrent or persistent udder infections and treatment failure through increased resistance to antibiotics and protection against host defences. In the present study 252 bacterial isolates from milk samples from bovine udder quarters with intramammary infections were examined with Congo Red agar (CRA) method and tube method (TM) for their ability to form biofilms. Both tests revealed a high number of biofilm-positive strains. Literature reports that the cure rates for Staphylococcus aureus infected udders are lower (27%) in comparison to cure rates of Streptococcus uberis (64-81%) or coagulase-negative staphylococci (CNS) mastitis (80-90%). The findings of the present study suggest that biofilm formation is not the main factor for the differences in cure rates of the various bacteria genera, because all tested pathogen groups showed a similarly high proportion of biofilm formation. Further research is needed to detect microbial biofilms on bovine udder epithelia.
KW - Animals
KW - Bacterial Physiological Phenomena
KW - Biofilms/growth & development
KW - Cattle
KW - Escherichia coli/physiology
KW - Female
KW - Klebsiella/physiology
KW - Mammary Glands, Animal/microbiology
KW - Mastitis, Bovine/drug therapy
KW - Microbiological Techniques/methods
KW - Milk/microbiology
KW - Staphylococcus/physiology
KW - Staphylococcus aureus/physiology
KW - Streptococcus/physiology
U2 - 10.1017/S0022029917000218
DO - 10.1017/S0022029917000218
M3 - Journal article
C2 - 28524019
VL - 84
SP - 198
EP - 201
JO - Journal of Dairy Research
JF - Journal of Dairy Research
SN - 0022-0299
IS - 2
ER -
ID: 237051370