Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine
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Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine. / Poulsen, L K; Nolte, H; Søndergaard, I; Stahl Skov, P.
In: Agents and Actions, Vol. 30, No. 3-4, 06.1990, p. 313-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine
AU - Poulsen, L K
AU - Nolte, H
AU - Søndergaard, I
AU - Stahl Skov, P
PY - 1990/6
Y1 - 1990/6
N2 - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.
AB - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.
KW - Adsorption
KW - Animals
KW - Antibody Specificity
KW - Cross Reactions
KW - Histamine
KW - Humans
KW - Immunization
KW - Immunoradiometric Assay
KW - Immunosorbents
KW - Iodine Radioisotopes
KW - Rabbits
KW - Serum Albumin
KW - Staphylococcal Protein A
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 2386106
VL - 30
SP - 313
EP - 318
JO - Agents and Actions
JF - Agents and Actions
SN - 0065-4299
IS - 3-4
ER -
ID: 169715257