Immune function and phenotype before and after highly active antiretroviral therapy
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Immune function and phenotype before and after highly active antiretroviral therapy. / Søndergaard, S R; Aladdin, H; Ullum, H; Gerstoft, J; Skinhøj, P; Pedersen, Bente Klarlund.
In: Journal of acquired immune deficiency syndromes (1999), Vol. 21, No. 5, 15.08.1999, p. 376-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Immune function and phenotype before and after highly active antiretroviral therapy
AU - Søndergaard, S R
AU - Aladdin, H
AU - Ullum, H
AU - Gerstoft, J
AU - Skinhøj, P
AU - Pedersen, Bente Klarlund
PY - 1999/8/15
Y1 - 1999/8/15
N2 - Immune functions represented by equal CD4 counts before and after highly active antiretroviral therapy (i.e., pre- and post-HAART) in the same HIV-infected patients, were examined. Twelve HIV-infected patients were included. Patients had equal CD4 counts pre- and post-HAART and were studied on average 30 months pre-HAART and 17 months post-HAART. Post-HAART, CD8+ T cells expressed greater amounts of CD28 (p < .02), smaller amounts of CD38 (p < .02), and a reduced proportion of CD4+CD28+ T cells expressed CD38+ (p < .01). Proliferation increased (p < 10) in lymphocyte cell cultures stimulated with pokeweed mitogens or Candida, and was correlated to expression of CD28 on T cells (p < .02). The proportion of CD3-CD16-CD56+ natural killer (NK) cells increased (p < .05) and CD3-CD16+CD56- NK cells declined (p < .01). Production of interferon-gamma increased (p < .10). The number of naive and memory T cells, the non-major histocompatibility complex (non-MHC)-restricted and HIV-specific MHC-restricted cytotoxicity and the production of macrophage inflammatory protein-1gamma were unchanged. The finding of increased expression of CD28, correlating to increased proliferation capacity, and diminished expression of CD38 on T cells, indicates that following long-term HAART, repopulation occurs with less activated cells with increased proliferative capacity. This finding may be of clinical importance in considering risk and vulnerability for progression of opportunistic infections post-HAART.
AB - Immune functions represented by equal CD4 counts before and after highly active antiretroviral therapy (i.e., pre- and post-HAART) in the same HIV-infected patients, were examined. Twelve HIV-infected patients were included. Patients had equal CD4 counts pre- and post-HAART and were studied on average 30 months pre-HAART and 17 months post-HAART. Post-HAART, CD8+ T cells expressed greater amounts of CD28 (p < .02), smaller amounts of CD38 (p < .02), and a reduced proportion of CD4+CD28+ T cells expressed CD38+ (p < .01). Proliferation increased (p < 10) in lymphocyte cell cultures stimulated with pokeweed mitogens or Candida, and was correlated to expression of CD28 on T cells (p < .02). The proportion of CD3-CD16-CD56+ natural killer (NK) cells increased (p < .05) and CD3-CD16+CD56- NK cells declined (p < .01). Production of interferon-gamma increased (p < .10). The number of naive and memory T cells, the non-major histocompatibility complex (non-MHC)-restricted and HIV-specific MHC-restricted cytotoxicity and the production of macrophage inflammatory protein-1gamma were unchanged. The finding of increased expression of CD28, correlating to increased proliferation capacity, and diminished expression of CD38 on T cells, indicates that following long-term HAART, repopulation occurs with less activated cells with increased proliferative capacity. This finding may be of clinical importance in considering risk and vulnerability for progression of opportunistic infections post-HAART.
KW - Anti-HIV Agents
KW - Antigens, CD
KW - Biomarkers
KW - CD4 Lymphocyte Count
KW - Cells, Cultured
KW - Disease Progression
KW - Drug Therapy, Combination
KW - Follow-Up Studies
KW - HIV Infections
KW - HIV Protease Inhibitors
KW - Humans
KW - Immunophenotyping
KW - Lymphocyte Activation
KW - Male
KW - T-Lymphocyte Subsets
KW - Time Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 10458618
VL - 21
SP - 376
EP - 383
JO - J A I D S
JF - J A I D S
SN - 1525-4135
IS - 5
ER -
ID: 180572112