Identifying Biomarkers for Osteogenic Potency Assay Development
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Identifying Biomarkers for Osteogenic Potency Assay Development. / Burns, Jorge S.; Kassem, Moustapha.
Potency Assays for Advanced Stem Cell Therapy Medicinal Products. ed. / Jorge S. Burns. Springer, 2023. p. 39-58 (Advances in Experimental Medicine and Biology, Vol. 1420).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Identifying Biomarkers for Osteogenic Potency Assay Development
AU - Burns, Jorge S.
AU - Kassem, Moustapha
N1 - Publisher Copyright: © 2023, Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - There has been extensive exploration of how cells may serve as advanced therapy medicinal products to treat skeletal pathologies. Osteoblast progenitors responsible for production of extracellular matrix that is subsequently mineralized during bone formation have been characterised as a rare bone marrow subpopulation of cell culture plastic adherent cells. Conveniently, they proliferate to form single-cell derived colonies of fibroblastoid cells, termed colony forming unit fibroblasts that can subsequently differentiate to aggregates resembling small areas of cartilage or bone. However, donor heterogeneity and loss of osteogenic differentiation capacity during extended cell culture have made the discovery of reliable potency assay biomarkers difficult. Nonetheless, functional osteoblast models derived from telomerised human bone marrow stromal cells have allowed extensive comparative analysis of gene expression, microRNA, morphological phenotypes and secreted proteins. This chapter highlights numerous insights into the molecular mechanisms underpinning osteogenic differentiation of multipotent stromal cells and bone formation, discussing aspects involved in the choice of useful biomarkers for functional attributes that can be quantitively measured in osteogenic potency assays.
AB - There has been extensive exploration of how cells may serve as advanced therapy medicinal products to treat skeletal pathologies. Osteoblast progenitors responsible for production of extracellular matrix that is subsequently mineralized during bone formation have been characterised as a rare bone marrow subpopulation of cell culture plastic adherent cells. Conveniently, they proliferate to form single-cell derived colonies of fibroblastoid cells, termed colony forming unit fibroblasts that can subsequently differentiate to aggregates resembling small areas of cartilage or bone. However, donor heterogeneity and loss of osteogenic differentiation capacity during extended cell culture have made the discovery of reliable potency assay biomarkers difficult. Nonetheless, functional osteoblast models derived from telomerised human bone marrow stromal cells have allowed extensive comparative analysis of gene expression, microRNA, morphological phenotypes and secreted proteins. This chapter highlights numerous insights into the molecular mechanisms underpinning osteogenic differentiation of multipotent stromal cells and bone formation, discussing aspects involved in the choice of useful biomarkers for functional attributes that can be quantitively measured in osteogenic potency assays.
KW - Biomarker correlation
KW - Bone formation
KW - Bone marrow
KW - Donor heterogeneity
KW - Functional attribute
KW - Global microRNA profiling
KW - Osteogenic differentiation
KW - Osteogenic potency assay
KW - Proteomic
KW - Telomerised MSC
U2 - 10.1007/978-3-031-30040-0_4
DO - 10.1007/978-3-031-30040-0_4
M3 - Book chapter
C2 - 37258783
AN - SCOPUS:85160765424
SN - 978-3-031-30039-4
SN - 978-3-031-30042-4
T3 - Advances in Experimental Medicine and Biology
SP - 39
EP - 58
BT - Potency Assays for Advanced Stem Cell Therapy Medicinal Products
A2 - Burns, Jorge S.
PB - Springer
ER -
ID: 390449309