Identification of new proteins in mature sieve elements
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Identification of new proteins in mature sieve elements. / Sanden, Niels Christian; Schulz, Alexander.
In: Physiologia Plantarum, Vol. 174, No. 1, e13634, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of new proteins in mature sieve elements
AU - Sanden, Niels Christian
AU - Schulz, Alexander
N1 - Publisher Copyright: © 2022 Scandinavian Plant Physiology Society.
PY - 2022
Y1 - 2022
N2 - The phloem enables vascular plants to transport photoassimilates from source tissues to heterotrophic sink tissues. In the phloem, unbroken strings of enucleated sieve elements, which lose the majority of their cellular contents upon maturation, provide a low resistance path for mass flow. The protein machinery in mature sieve elements performs vital functions to maintain the flow, transmit systemic signals and defend the sugar stream against pests. However, our knowledge of this particular protein population is very limited since mature sieve elements are difficult to isolate and not amenable to transcriptomic analysis due to their enucleate nature. Here, we used co-expression analysis and published gene clusters from transcriptomic studies to generate a list of sieve element proteins that potentially survive the enucleation process to reside in mature sieve elements. We selected seven candidates and show that they all localize in sieve elements in Arabidopsis roots and six of them in bolting stems. Our results support the idea that nascent sieve elements prior to enucleation translate part of the protein machinery found in mature sieve elements. Our co-expression list and the publicly available gene clusters expressed in late proto- and meta-phloem sieve elements are valuable resources for uncharacterized genes that may function in mature sieve elements.
AB - The phloem enables vascular plants to transport photoassimilates from source tissues to heterotrophic sink tissues. In the phloem, unbroken strings of enucleated sieve elements, which lose the majority of their cellular contents upon maturation, provide a low resistance path for mass flow. The protein machinery in mature sieve elements performs vital functions to maintain the flow, transmit systemic signals and defend the sugar stream against pests. However, our knowledge of this particular protein population is very limited since mature sieve elements are difficult to isolate and not amenable to transcriptomic analysis due to their enucleate nature. Here, we used co-expression analysis and published gene clusters from transcriptomic studies to generate a list of sieve element proteins that potentially survive the enucleation process to reside in mature sieve elements. We selected seven candidates and show that they all localize in sieve elements in Arabidopsis roots and six of them in bolting stems. Our results support the idea that nascent sieve elements prior to enucleation translate part of the protein machinery found in mature sieve elements. Our co-expression list and the publicly available gene clusters expressed in late proto- and meta-phloem sieve elements are valuable resources for uncharacterized genes that may function in mature sieve elements.
U2 - 10.1111/ppl.13634
DO - 10.1111/ppl.13634
M3 - Journal article
C2 - 35060148
AN - SCOPUS:85125894260
VL - 174
JO - Physiologia Plantarum
JF - Physiologia Plantarum
SN - 0031-9317
IS - 1
M1 - e13634
ER -
ID: 300453136