High-throughput siRNA screening applied to the ubiquitin-proteasome system
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.
Original language | English |
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Title of host publication | Proteostasis |
Editors | Rune Matthiesen |
Number of pages | 19 |
Publisher | Springer |
Publication date | 2016 |
Pages | 421-439 |
Chapter | 28 |
ISBN (Print) | 978-1-4939-3754-7 |
ISBN (Electronic) | 978-1-4939-3756-1 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1449 |
ISSN | 1064-3745 |
- Journal Article
Research areas
ID: 179133797