HGF is released from buccal fibroblasts after smokeless tobacco stimulation
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HGF is released from buccal fibroblasts after smokeless tobacco stimulation. / Dabelsteen, S; Christensen, S; Gron, B; Bardow, A; Dabelsteen, Erik.
In: Oral Oncology, Vol. 41, No. 5, 2005, p. 509-14.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - HGF is released from buccal fibroblasts after smokeless tobacco stimulation
AU - Dabelsteen, S
AU - Christensen, S
AU - Gron, B
AU - Bardow, A
AU - Dabelsteen, Erik
PY - 2005
Y1 - 2005
N2 - To investigate the effect of smokeless tobacco (ST) on (1) HGF, KGF and GM-CSF expression by buccal fibroblasts and (2) on keratinocyte and fibroblast proliferation. Buccal fibroblasts were stimulated with different concentrations of ST extracts in a double dilution from 0.50% w/v to 0.03% w/v. Supernatant was collected after 24/48/72/96 h and assayed for HGF, KGF, and GM-CSF by ELISA. The amount of RNA was used as an indicator of fibroblast cell number. Buccal epithelial cell proliferation was determined by CyQUANT proliferation assay. The amount of HGF and KGF in the supernatant was dependent on exposure time and on concentration of the tobacco extract. High concentration increased production of HGF 4-fold. KGF production was doubled when high concentration of tobacco was used, low concentration did not stimulate cells. GM-CSF production was low in both stimulated and non-stimulated cells. Keratinocytes and fibroblasts showed no increase in proliferation after stimulation with increased concentrations of ST. The results suggest that HGF and KGF may play an important role as a paracrine growth factor in epithelial hyperplasia in ST lesions.
AB - To investigate the effect of smokeless tobacco (ST) on (1) HGF, KGF and GM-CSF expression by buccal fibroblasts and (2) on keratinocyte and fibroblast proliferation. Buccal fibroblasts were stimulated with different concentrations of ST extracts in a double dilution from 0.50% w/v to 0.03% w/v. Supernatant was collected after 24/48/72/96 h and assayed for HGF, KGF, and GM-CSF by ELISA. The amount of RNA was used as an indicator of fibroblast cell number. Buccal epithelial cell proliferation was determined by CyQUANT proliferation assay. The amount of HGF and KGF in the supernatant was dependent on exposure time and on concentration of the tobacco extract. High concentration increased production of HGF 4-fold. KGF production was doubled when high concentration of tobacco was used, low concentration did not stimulate cells. GM-CSF production was low in both stimulated and non-stimulated cells. Keratinocytes and fibroblasts showed no increase in proliferation after stimulation with increased concentrations of ST. The results suggest that HGF and KGF may play an important role as a paracrine growth factor in epithelial hyperplasia in ST lesions.
KW - Adult
KW - Cell Proliferation
KW - Cells, Cultured
KW - Cheek
KW - Dose-Response Relationship, Drug
KW - Enzyme-Linked Immunosorbent Assay
KW - Fibroblast Growth Factor 7
KW - Fibroblast Growth Factors
KW - Fibroblasts
KW - Granulocyte-Macrophage Colony-Stimulating Factor
KW - Hepatocyte Growth Factor
KW - Humans
KW - Keratinocytes
KW - Mouth Mucosa
KW - Tobacco, Smokeless
U2 - 10.1016/j.oraloncology.2004.12.011
DO - 10.1016/j.oraloncology.2004.12.011
M3 - Journal article
C2 - 15878756
VL - 41
SP - 509
EP - 514
JO - Oral Oncology Extra
JF - Oral Oncology Extra
SN - 1741-9409
IS - 5
ER -
ID: 119538255